Teleost isotocin receptor: structure, functional expression, mRNA distribution and phylogeny
| Autor: | Karl Lederis, Henk Zwiers, Dietmar Richter, Wolfgang Meyerhof, Holger Hausmann |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
White sucker
Vasopressin receptor Molecular Sequence Data Urinary Bladder Hypothalamus Biophysics Vasotocin Biology Oxytocin Biochemistry Conserved sequence Xenopus laevis chemistry.chemical_compound Structural Biology Complementary DNA Genetics Animals Amino Acid Sequence RNA Messenger Cloning Molecular Receptor Oxytocin receptor Molecular Biology Phylogeny Brain Chemistry Messenger RNA Base Sequence Myocardium Ovary Fishes Cell Biology Cell biology Intestines Neuropeptide Transmembrane domain Liver chemistry Receptors Oxytocin Catostomus commersoni Oocytes Female Spleen |
| Zdroj: | FEBS Letters. 370:227-230 |
| ISSN: | 0014-5793 |
| Popis: | A cDNA encoding a receptor for the oxytocin-related peptide isotocin has been identified by screening a lambda gt11 library constructed from poly(A)+ RNA of the hypothalamic region of the teleost Catostomus commersoni. The probe used was obtained by PCR amplification of white sucker genomic DNA using degenerate primers based on conserved sequences in the mammalian receptor counterparts. The full-length cDNA specifies a polypeptide of 390 amino acid residues that displays the typical hydrophobicity profile of a seven transmembrane domain receptor and which exhibits greatest similarity to mammalian oxytocin receptors. Oocytes that express the cloned receptor respond to the application of isotocin by an induction of membrane chloride currents indicating that it is coupled to the inositol phosphate/calcium pathway. The isotocin receptor (ITR) can also be activated by vasotocin, mesotocin, oxytocin and Arg-vasopressin, although these have lower potencies than isotocin. ITR-encoding mRNA has been detected in brain, intestine, bladder, skeletal muscle, lateral line, gills and kidney indicating that this receptor may mediate a variety of physiological functions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text