Do Deletions of Mos1-Like Elements Occur Randomly in the Drosophilidae Family?
| Autor: | Fabienne Godin, Frédéric Brunet, Pierre Capy, Tatiana Giraud |
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| Rok vydání: | 2002 |
| Předmět: |
Transposable element
DNA Repair Molecular Sequence Data Population Biology Drosophila mauritiana Drosophilidae Genetics Consensus sequence Animals Direct repeat Ectopic recombination education Molecular Biology Mauritiana Ecology Evolution Behavior and Systematics Repetitive Sequences Nucleic Acid Sequence Deletion Recombination Genetic education.field_of_study Base Sequence Sequence Analysis DNA biology.organism_classification DNA Transposable Elements |
| Zdroj: | Journal of Molecular Evolution. 54:227-234 |
| ISSN: | 1432-1432 0022-2844 |
| Popis: | We compared deleted copies of the seven mauritiana subfamilies of mariner transposable elements in species of the Drosophilidae. All elements were detected by PCR using the inverted terminal repeats of the Mos1 element of Drosophila mauritiana as primers. A higher frequency of breakpoints in the 5prime prime or minute part of the element compared to the 3prime prime or minute part was observed. Of the 27 deletions, 9 (33%) occurred between short direct repeats (SDR) of 5 to 8 bp. The SDRs can be at or close to the breakpoints of the deletion. A deleted copy of D. simulans (St. Martin population) had three repeats of a motif present only once in the complete consensus sequence. The high frequency of SDRs at or near the breakpoints of the deletions strongly suggests that some of them do not occur at random. Mechanisms that might explain these deletions, such as unequal crossing-over, ectopic recombination, and abortive gap repair, are discussed. |
| Databáze: | OpenAIRE |
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