Fibronectin-binding protein TDE1579 affects cytotoxicity of Treponema denticola
| Autor: | Margarita Mikhailova, Ryan Montgomery, Bjorn Steffensen, Lianrui Chu, Xiaoping Xu, Veronica J. Lai, Trista K. Robichaud |
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| Rok vydání: | 2015 |
| Předmět: |
Mutant
Plasma protein binding Gene Mutant Cell morphology Microbiology Article law.invention stomatognathic system Bacterial Proteins law Humans biology Treponemal Infections Virulence Treponema denticola biology.organism_classification Molecular biology Fibronectins Fibronectin stomatognathic diseases Kinetics Infectious Diseases Fibronectin binding Recombinant DNA biology.protein Protein Binding |
| Zdroj: | Anaerobe. 36 |
| ISSN: | 1095-8274 |
| Popis: | While FbpA, a family of bacterial fibronectin (FN) binding proteins has been studied in several gram-positive bacteria, the gram-negative Treponema denticola, an anaerobic periodontal pathogen, also has an overlooked fbp gene (tde1579). In this research, we confirm that recombinant Fbp protein (rFbp) of T. denticola binds human FN with a Kdapp of 1.5 × 10(-7) M and blocks the binding of T. denticola to FN in a concentration-dependent manner to a level of 42%. The fbp gene was expressed in T. denticola. To reveal the roles of fbp in T. denticola pathogenesis, an fbp isogenic mutant was constructed. The fbp mutant had 51% reduced binding ability to human gingival fibroblasts (hGF). When hGF were challenged with T. denticola, the fbp mutant caused less cell morphology change, had 50% reduced cytotoxicity to hGF, and had less influence on the growth of hGF cells. |
| Databáze: | OpenAIRE |
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