Effect of different freeze-thaw regimes on ice formation and ultrastructural changes in tomato fruit parenchyma tissue
| Autor: | M. Stein, W.P. Mohr |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Cell Nucleus
Cytoplasm Protein Denaturation Ice formation Membranes Ice crystals Histological Techniques General Medicine Biology Plant cell General Biochemistry Genetics and Molecular Biology Protoplasm Cell wall Microscopy Electron Cell Wall Phase (matter) Fruit Plant Cells Parenchyma Botany Freezing Ultrastructure Biophysics General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 6(1) |
| ISSN: | 0011-2240 |
| Popis: | Summary The effect of different freeze-thaw regimes on the ultrastructure of tomato fruit parenchyma tissue has been presented. Ice patterns formed as a result of the freezing phase were also determined, using freeze-substitution and freeze-etching techniques. Fine structure of these highly vacuolated, high moisture cells typically comprising most plant tissues was extensively altered by all freeze-thaw combinations tried. Membranous components of the protoplasm were especially affected. Nevertheless, apparent damage was minimal following intermediate rates of freezing and thawing. A possible explanation based on the size of ice crystals formed during freezing was discussed. The general results were discussed in relation to freeze-thaw-induced ultrastructural changes that have been reported for animal cells and tissues. |
| Databáze: | OpenAIRE |
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