Comparision of the BAX® System with an in-house MSRV method for the detection of Salmonella in chicken carcasses and pork meat
| Autor: | Dalton Francisco de Andrade, Giovana Lemos, Cleide Rosana Vieira Batista, Maura Chiapinoto, Ivair G. da Silva, Paulo José Ogliari, Paulo Rogério Franchin, Marina Rebelatto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Salmonella
animal structures Sistema BAX® Método MSRV MSRV method medicine.medical_treatment chicken Pcr assay Significant difference technology industry and agriculture food and beverages Biology medicine.disease_cause Microbiology pork meat frango carne suína Pork meat medicine Food science BAX® System Evisceration (ophthalmology) |
| Zdroj: | Brazilian Journal of Microbiology, Volume: 37, Issue: 4, Pages: 521-526, Published: DEC 2006 Brazilian Journal of Microbiology v.37 n.4 2006 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| Popis: | A study was performed to compare the analytical procedure of the BAX® System for Salmonella PCR assay with the Modified Semi-Solid Rappaport-Vassiliadis (MSRV) method, for the detection of Salmonella in naturally contaminated chicken carcass samples (n = 762) and raw pork meat (n = 566). The chicken carcasses samples were collected during slaughtering after defeathering or immediately after evisceration and the raw pork meat collected from the deboned head of recently slaughtered pigs and others deboned raw fresh pork meat. The BAX® System detected 134 Salmonella-positive samples in chicken carcasses and 145 samples in pork meat, while the MSRV method isolated 142 and 144 Salmonella-positive samples, respectively. No significant difference was observed between the two methods for chicken carcasses and pork meat, according to McNemar test at the 5% level. Um estudo foi realizado com o objetivo de comparar o procedimento analítico de detecção de Salmonella com o Sistema BAX® automatizado, baseado na Reação em Cadeia da Polimerase (PCR) com o método de Rappaport-Vassiliadis em Agar Semi-Sólido modificado (MSRV) para detecção de Salmonella em amostras de carcaças de frango naturalmente contaminadas (n=762) e retalhos de carne suía (n=566). O Sistema BAX® detectou 134 amostras positivas para Salmonella em carcaças de frango e 145 amostras positivas para Salmonella em retalhos de carne suína, enquanto o MSRV detectou 142 e 144 amostras positivas respectivamente. Não houve diferença estatisticamente significativa entre os dois métodos, segundo McNemar ao nível de significância de 5%. |
| Databáze: | OpenAIRE |
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