Local Pheromone Release from Dynamic Polarity Sites Underlies Cell-Cell Pairing during Yeast Mating
| Autor: | Sophie G. Martin, Felipe O. Bendezú, Laura Merlini, Bita Khalili, Daniel Hurwitz, Dimitrios Vavylonis, Vincent Vincenzetti |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
education Biophysics CDC42 Saccharomyces cerevisiae Biology Pheromones General Biochemistry Genetics and Molecular Biology Article Cell Fusion 03 medical and health sciences Botany Cell polarity Schizosaccharomyces ATP-Binding Cassette Transporters/metabolism Cell Polarity Pheromones/metabolism Schizosaccharomyces/cytology Schizosaccharomyces/physiology Schizosaccharomyces pombe Proteins/chemistry Schizosaccharomyces pombe Proteins/metabolism Signal Transduction cdc42 GTP-Binding Protein/metabolism cdc42 GTP-Binding Protein health care economics and organizations Cell Membrane biology.organism_classification Cell biology 030104 developmental biology Mating of yeast Pairing Sex pheromone Schizosaccharomyces pombe Pheromone ATP-Binding Cassette Transporters Schizosaccharomyces pombe Proteins Signal transduction General Agricultural and Biological Sciences |
| Zdroj: | Current biology, vol. 26, no. 8, pp. 1117-1125 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2015.11.817 |
| Popis: | Cell pairing is central for many processes, including immune defense, neuronal connection, hyphal fusion, and sexual reproduction. How does a cell orient toward a partner, especially when faced with multiple choices? Fission yeast Schizosaccharomyces pombe P and M cells, which respectively express P and M factor pheromones [1, 2], pair during the mating process induced by nitrogen starvation. Engagement of pheromone receptors Map3 and Mam2 [3, 4] with their cognate pheromone ligands leads to activation of the Gα protein Gpa1 to signal sexual differentiation [3, 5, 6]. Prior to cell pairing, the Cdc42 GTPase, a central regulator of cell polarization, forms dynamic zones of activity at the cell periphery at distinct locations over time [7]. Here we show that Cdc42-GTP polarization sites contain the M factor transporter Mam1, the general secretion machinery, which underlies P factor secretion, and Gpa1, suggesting that these are sub-cellular zones of pheromone secretion and signaling. Zone lifetimes scale with pheromone concentration. Computational simulations of pair formation through a fluctuating zone show that the combination of local pheromone release and sensing, short pheromone decay length, and pheromone-dependent zone stabilization leads to efficient pair formation. Consistently, pairing efficiency is reduced in the absence of the P factor protease. Similarly, zone stabilization at reduced pheromone levels, which occurs in the absence of the predicted GTPase-activating protein for Ras, leads to reduction in pairing efficiency. We propose that efficient cell pairing relies on fluctuating local signal emission and perception, which become locked into place through stimulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|