Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing Analysis
| Autor: | Jiaxi Zhang, Xiaodong Liu, Fengzhi Wu, Haonan Fu, Xueqin Yang, Ling Zhou, Yinjia He, Lanying Liu, Li Zhang, Rong Zhang, Qiuyi Lv, Linqing Miao, Xiulun Gan, Anming Liu, Jie Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
GB20 (Fengchi)
Pathology medicine.medical_specialty genetic structures medicine.medical_treatment Cognitive Neuroscience Neural degeneration Retinal ganglion lcsh:RC346-429 050105 experimental psychology lcsh:RC321-571 03 medical and health sciences chemistry.chemical_compound Cellular and Molecular Neuroscience GV16 (Fengfu) 0302 clinical medicine medicine Acupuncture 0501 psychology and cognitive sciences lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry lcsh:Neurology. Diseases of the nervous system Original Research ATF3 Retina business.industry 05 social sciences RNA sequencing Retinal BL1 (Jingming) optic nerve crush eye diseases Sensory Systems medicine.anatomical_structure retinal ganglion cells chemistry Optic nerve sense organs Axotomy business transcriptome acupuncture 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Integrative Neuroscience, Vol 13 (2019) Frontiers in Integrative Neuroscience |
| ISSN: | 1662-5145 |
| DOI: | 10.3389/fnint.2019.00059 |
| Popis: | Glaucoma and traumatic optic nerve crush (ONC) injury result in progressive loss of retinal ganglion cells (RGCs) and defects in visual function. In clinical trials of Traditional Chinese Medicine, acupuncture has been widely used for the treatment of ocular diseases. However, the molecular mechanisms of acupuncture treatment are still unclear. In this study, we used technique of RNA sequencing (RNA-seq) to study the effects of acupuncture treatment on retinal transcriptome after axotomy injury. RNA-seq results revealed that 436 genes including 31 transcription factors (TFs) were changed after injury, among them were many well-known neural degeneration related TFs such as Jun, Ddit3, Atf3, and Atf4. Interestingly, acupuncture treatment at acupoint GB20 (Fengchi) significantly reversed a series of differential expressed genes (DEGs) induced by optic nerve injury. While treatments at BL1 (Jingming) or GB20 sham control acupoint-GV16 (Fengfu), led to limited DEG reversal. In contrast, treatments at these two sites further enhanced the trend of DEG expression induced by axotomy injury. At last, retina immunostaining results revealed that only GB20 acupoint treatment increased RGC survival, in consistent with RNA-seq results. Therefore, our study first reported that acupuncture treatment regulated retinal transcriptome and reversed the gene expression induced by axotomy injury, and GB20 acupoint treatment increased RGC survival, which will provide novel therapeutic targets for treatment of ocular diseases. |
| Databáze: | OpenAIRE |
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