Antimicrobials as Single and Combination Therapy for Colistin-Resistant Pseudomonas aeruginosa at a University Hospital in Thailand
| Autor: | Jantima Traipattanakul, Wichai Santimaleeworagun, Sudaluck Thunyaharn, Supanun Pungcharoenkijkul |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Imipenem mcr-1 030106 microbiology Ceftazidime minimal inhibitory concentration Biochemistry Microbiology Tazobactam Meropenem Article carbapenemase 03 medical and health sciences 0302 clinical medicine polycyclic compounds Medicine Pharmacology (medical) cardiovascular diseases 030212 general & internal medicine General Pharmacology Toxicology and Pharmaceutics business.industry lcsh:RM1-950 biochemical phenomena metabolism and nutrition bacterial infections and mycoses respiratory tract diseases lcsh:Therapeutics. Pharmacology Infectious Diseases Amikacin biofilm formation Colistin lipids (amino acids peptides and proteins) Ceftolozane business medicine.drug Piperacillin |
| Zdroj: | Antibiotics Volume 9 Issue 8 Antibiotics, Vol 9, Iss 475, p 475 (2020) |
| ISSN: | 2079-6382 |
| Popis: | Global infections with colistin-resistant Pseudomonas aeruginosa (CoR-PA) are increasing there are currently very few studies focused on the antimicrobial susceptibility of CoR-PA isolates, and none from Thailand. Here, we investigated the impact of various antimicrobials, alone and in combination, via the in vitro testing of CoR-PA clinical isolates. Eighteen CoR-PA isolates were obtained from patients treated at Phramongkutklao Hospital from January 2010 through June 2019 these were classified into six different clonal types by using the enterobacterial repetitive intergenic consensus (ERIC)-PCR method, with a high prevalence of Group A (27.8%). The antimicrobial susceptibility was determined as the minimal inhibitory concentrations (MICs) using the epsilometer-test (E-test) method. The synergistic activities of six antimicrobial combinations were reported via the fractional-inhibitory-concentration index. All CoR-PA isolates were susceptible to amikacin, meropenem, and ceftolozane/tazobactam, but only 5.56% were susceptible to imipenem. In vitro synergistic activities were detected for amikacin with aztreonam, piperacillin/tazobactam, meropenem, and ceftazidime for 16.67%, 11.11%, 11.11%, and 5.55%, respectively. One CoR-PA isolate carried the blaVIM metallo-&beta lactamase gene none carried mcr-1 genes or detected plasmid-mediated AmpC &beta lactamase or an overproduction of chromosomal AmpC &beta lactamase. Seven CoR-PA isolates (38.89%) were capable of biofilm formation. In conclusion, CoR-PA isolates are highly susceptible to antimicrobials the synergy observed in response to the various agents should be examined in a clinical setting. |
| Databáze: | OpenAIRE |
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