Brazilian Green Propolis Extract Synergizes with Protoporphyrin IX-mediated Photodynamic Therapy via Enhancement of Intracellular Accumulation of Protoporphyrin IX and Attenuation of NF-κB and COX-2
| Autor: | Chengcheng Wang, He Ling, Yuxuan Wang, Liao Youwei, Chen Xingguang, Jing Yao, Nian-Qin Yu, Hu Die, Xiaoyan Wang, Wang Hao, Liang Wu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Pharmaceutical Science Protoporphyrins synergy Photodynamic therapy Analytical Chemistry HeLa chemistry.chemical_compound 0302 clinical medicine Annexin Drug Discovery Brazilian green propolis photodynamic therapy cancer inflammation apoptosis Chromatography High Pressure Liquid Mice Inbred BALB C Protoporphyrin IX NF-kappa B Drug Synergism Flow Cytometry Biochemistry Chemistry (miscellaneous) 030220 oncology & carcinogenesis Molecular Medicine Heterografts A431 cells Brazil Mice Nude Biology Propolis Article lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry In vivo Cell Line Tumor medicine Animals Humans Physical and Theoretical Chemistry Organic Chemistry biology.organism_classification In vitro 030104 developmental biology chemistry Photochemotherapy Apoptosis Cyclooxygenase 2 Cancer research Spectrophotometry Ultraviolet |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules, Vol 22, Iss 5, p 732 (2017) Molecules; Volume 22; Issue 5; Pages: 732 |
| ISSN: | 1420-3049 |
| Popis: | Brazilian green propolis (BGP) is noted for its impressive antitumor effects and has been used as a folk medicine in various cultures for many years. It has been demonstrated that BGP could enhance the cytotoxic effect of cytostatic drugs on tumor cells. Photodynamic therapy (PDT) is a therapeutic approach used against malignant cells. To assess the synergistic effect of BGP extract on protoporphyrin IX (PpIX)-mediated photocytotoxicity, MTT assays were performed using A431 and HeLa cells. TUNEL assay and Annexin V-FITC/PI staining were performed to confirm the induction of apoptosis. Western blotting analysis was performed to examine the pro-apoptotic proteins, anti-apoptotic proteins and inflammation related proteins in A431 cells. Intracellular accumulation of PpIX was examined by flow cytometry. The synergistic effect of BGP extract in PpIX-PDT was also evaluated with a xenograft model. Our findings reveal that BGP extract increased PpIX-mediated photocytotoxicity in A431 and HeLa cells. PpIX-PDT with BGP extract treatment resulted in a decrease in Bcl-xL and an increase in NOXA, Bax and caspase-3 cleavage. The protein expression levels of p-IKKα/β, NF-κB and COX-2 were upregulated by PpIX-PDT but significantly attenuated when in combination with BGP extract. BGP extract was also found to significantly enhance the intracellular accumulation of PpIX in A431 cells. BGP extract increased PpIX-mediated photocytotoxicity in a xenograft model as well. Our findings provide evidence for a synergistic effect of BGP extract in PpIX-PDT both in vitro and in vivo. |
| Databáze: | OpenAIRE |
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