Neutralizing IGF-1 Monoclonal Antibody With Cross-Species Reactivity
| Autor: | C Edwards, S Stimpson, Jui-Lan Su, S Burgess, J Van Arnold, P Lin |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.drug_class
Immunology Cross Reactions Monoclonal antibody Binding Competitive Receptor IGF Type 1 law.invention Mice Dogs In vivo law Genetics medicine Animals Humans Insulin-Like Growth Factor I Receptor Mice Inbred BALB C biology Cell growth Goats Antibodies Monoclonal 3T3 Cells Molecular biology In vitro Rats Cell culture Cats Recombinant DNA biology.protein Cattle Antibody Cell Division |
| Zdroj: | Hybridoma. 16:513-518 |
| ISSN: | 0272-457X |
| Popis: | We report the generation of a murine IGF-1 monoclonal antibody designated 35I17, which exhibits unique cross-species reactivity. The antibody recognizes recombinant human and rat IGF-1 in ELISA, Western blots, and in an 125I-recombinant human IGF-1 Scintillation Proximity Assay. In addition, 35I17 blocks cell proliferation induced by recombinant human and rat IGF-1, and inhibits cell proliferation induced by sera from human, rat, calf, dog, goat, or mouse. The antibody inhibits rat IGF-1 binding to IGF-1 receptors, and prevents IGF-1-stimulated receptor and IRS-1 phosphorylation in LISN C4 cells, an IGF-1 receptor-transfected cell line. The cross-species and neutralizing properties of 35I17 may be useful in in vitro and in vivo animal studies for elucidating the role of IGF-1 in cancer, rheumatoid arthritis, and other diseases. |
| Databáze: | OpenAIRE |
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