Genetic address book for retinal cell types
| Autor: | Botond Roska, Nathaniel Heintz, Sandra Siegert, Brigitte Gross Scherf, Karina Del Punta, Nick Didkovsky |
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| Rok vydání: | 2009 |
| Předmět: |
Retinal Ganglion Cells
Genetically modified mouse Retinal Bipolar Cells Cell type Patch-Clamp Techniques Green Fluorescent Proteins Cell Mice Transgenic In Vitro Techniques Retinal Horizontal Cells Biology Retina Mice Species Specificity Night vision Neural Pathways medicine Animals Vision Ocular Systems neuroscience Neocortex General Neuroscience Immunohistochemistry Amacrine Cells medicine.anatomical_structure Microscopy Fluorescence Synapses Neuron Neuroscience Photoreceptor Cells Vertebrate |
| Zdroj: | Nature Neuroscience. 12:1197-1204 |
| ISSN: | 1546-1726 1097-6256 |
| DOI: | 10.1038/nn.2370 |
| Popis: | The mammalian brain is assembled from thousands of neuronal cell types that are organized in distinct circuits to perform behaviorally relevant computations. Transgenic mouse lines with selectively marked cell types would facilitate our ability to dissect functional components of complex circuits. We carried out a screen for cell type-specific green fluorescent protein expression in the retina using BAC transgenic mice from the GENSAT project. Among others, we identified mouse lines in which the inhibitory cell types of the night vision and directional selective circuit were selectively labeled. We quantified the stratification patterns to predict potential synaptic connectivity between marked cells of different lines and found that some of the lines enabled targeted recordings and imaging of cell types from developing or mature retinal circuits. Our results suggest the potential use of a stratification-based screening approach for characterizing neuronal circuitry in other layered brain structures, such as the neocortex. |
| Databáze: | OpenAIRE |
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