Monoclonal antibodies to a recombinant human leukocyte interferon (rIFN-αB)
| Autor: | P.L. Osheroff, S.M. Tahara, T.-R. Chiang |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Immunogen
medicine.drug_class Immunology Enzyme-Linked Immunosorbent Assay Cross Reactions Monoclonal antibody Pathology and Forensic Medicine law.invention Mice Western blot Antibody Specificity law Interferon medicine Animals Ascitic Fluid Humans Immunology and Allergy Mice Inbred BALB C Hybridomas biology medicine.diagnostic_test Human leukocyte interferon Antibodies Monoclonal Virology Molecular biology Molecular Weight Immunoassay Interferon Type I biology.protein Recombinant DNA Autoradiography Electrophoresis Polyacrylamide Gel Female Antibody medicine.drug |
| Zdroj: | Clinical Immunology and Immunopathology. 30:188-196 |
| ISSN: | 0090-1229 |
| Popis: | Recombinant human leukocyte B interferon (rIFN-alpha B) was purified to approximately 60% on a column of monoclonal antibody to IFN-alpha. This partially purified rIFN-alpha B was in turn used as immunogen in obtaining four monoclonal antibodies to this recombinant interferon. All four antibodies, while showing varying degrees of cross-reactivity to some other rIFN-alpha subtypes, showed specificity to monomeric and dimeric forms of rIFN-alpha B only and not to the contaminants in the partially purified preparation as demonstrated in the Western blot immunoassay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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