Infliximab alleviates the mortality, mesenteric hypoperfusion, aortic dysfunction, and multiple organ damage in septic rats
| Autor: | Ceyhan Ugurluoglu, Alper B. Iskit, Erdem Kamil Ozer, Hulagu Bariskaner, Mustafa Tugrul Goktas, Ibrahim Kilinc, Aysun Toker |
|---|---|
| Přispěvatelé: | Selçuk Üniversitesi, Farmakoloji |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine medicine.medical_specialty vascular reactivity Physiology Multiple Organ Failure multiple organ dysfunction medicine.disease_cause Gastroenterology survival Muscle Smooth Vascular Proinflammatory cytokine Sepsis sepsis 03 medical and health sciences Physiology (medical) Diabetes mellitus Internal medicine Medicine Animals Mesentery Pharmacology & Pharmacy Rats Wistar Aorta Pharmacology mesenteric arterial blood flow business.industry Interleukin-6 General Medicine medicine.disease Infliximab Rats stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Immunology Blood Circulation Tumor necrosis factor alpha Female business infliximab Perfusion Oxidative stress medicine.drug Artery Muscle Contraction |
| Popis: | WOS: 000404738500012 PubMed: 28459157 Tumor necrosis factor-alpha (TNF-alpha) is a pivotal mediator that triggers inflammatory process, oxidative stress, and multiple organ injury in sepsis. We investigated the effects of infliximab on survival, mesenteric artery blood flow (MBF), vascular reactivity, and oxidative and inflammatory injuries in cecal ligation and puncture (CLP)-induced sepsis. Wistar rats were divided into Sham, CLP, Sham+infliximab, and CLP+infliximab subgroups. Twenty-four hours before the operations, rats were injected intraperitoneally with infliximab (7 mg/kg) or vehicle (saline; 1 mL/kg). Twenty hours after the operations, MBF and phenylephrine responses of isolated aortic rings were measured. Tissue damages were examined biochemically and histopathologically. Furthermore, survival rates were monitored throughout 96 h. Infliximab improved survival, mesenteric perfusion, and aortic function after CLP. Increases of serum AST, ALT, LDH, BUN, Cr, and inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6) induced by CLP were blocked by infliximab. Infliximab prevented malondialdehyde elevations in septic liver, lung, spleen, and kidney tissues, as well as glutathione reductions in septic liver, spleen, and kidney tissues. Protective effects of infliximab on multiple organ damage were also observed histopathologically. Infliximab showed protective effects in sepsis due to its improvement effects on mesenteric perfusion, aortic function, and its anti-inflammatory and antioxidative effects. Turkish Academy of SciencesTurkish Academy of Sciences [EA-TUBA-GEBIP/2001-2-11] Alper B. Iskit has been supported by the Turkish Academy of Sciences, in the framework of the Young Scientist Award Program (EA-TUBA-GEBIP/2001-2-11). This study received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors. |
| Databáze: | OpenAIRE |
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