Determination of melamine in milk by fluorescence spectroscopy and second-order calibration
| Autor: | Matheus C. Barreto, Raíssa G. Braga, Wallace D. Fragoso, Sherlan G. Lemos |
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| Rok vydání: | 2021 |
| Předmět: |
Green chemistry
Food Contamination 01 natural sciences Fluorescence spectroscopy Analytical Chemistry Matrix (chemical analysis) Acetic acid chemistry.chemical_compound 0404 agricultural biotechnology Animals Solubility Chromatography Triazines Chemistry 010401 analytical chemistry 04 agricultural and veterinary sciences General Medicine 040401 food science 0104 chemical sciences Dilution Milk Spectrometry Fluorescence Calibration Melamine Food Science Fire retardant |
| Zdroj: | Food Chemistry. 364:130407 |
| ISSN: | 0308-8146 |
| Popis: | Melamine is a compound commonly used in the manufacturing of plastic and flame retardant products, but due to its solubility on water and high nitrogen content, it is also used to adulterate milk to mask adulteration by dilution in protein content tests. This work proposes a quick method using excitation-emission matrix (EEM) fluorescence spectroscopy and second-order calibration methods (PARAFAC and U-PLS/RBL) for the identification and quantification of melamine in milk. The proposed method uses a single clean-up step with acetic acid, resulting in a quick, low-cost, and environmentally friendly procedure, in agreement with green chemistry principles. Both PARAFAC and U-PLS/RBL were capable of detecting melamine in milk above 120.6 and 146.5 ppm respectively, adequate for adulterations above 2% in volume, with RMSEPs of 68.6 and 81.9 ppm, respectively. |
| Databáze: | OpenAIRE |
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