Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes

Autor: Simona Sovová, Z. Vargová, Petra Olejníková, Gabriela Kuzderová, Michaela Rendošová, Danica Sabolová, Simonida Stokič, Martin Kello, Róbert Gyepes, Mária Vilková, Ivana Bačová
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Chemical Phenomena
Molecular Conformation
Pharmaceutical Science
Organic chemistry
Chemistry Techniques
Synthetic
topoisomerase I inhibition
Crystallography
X-Ray
Analytical Chemistry
HeLa
chemistry.chemical_compound
QD241-441
Anti-Infective Agents
Drug Stability
Coordination Complexes
Drug Discovery
silver(I) complexes
Cytotoxicity
biology
Cell Cycle
Dipeptides
anticancer activity
Chemistry (miscellaneous)
cell cycle arrest
Thermogravimetry
Molecular Medicine
Selectivity
dipeptide
crystal structure
Silver
Stereochemistry
Antineoplastic Agents
Molecular Dynamics Simulation
Article
Cell cycle phase
Structure-Activity Relationship
Cell Line
Tumor
Humans
Physical and Theoretical Chemistry
Dipeptide
antimicrobial activity
Dose-Response Relationship
Drug
Topoisomerase
Spectrum Analysis
stability
biology.organism_classification
In vitro
DNA interaction
chemistry
biology.protein
DNA
Zdroj: Molecules
Molecules, Vol 26, Iss 6335, p 6335 (2021)
Volume 26
Issue 21
ISSN: 1420-3049
Popis: Three silver(I) dipeptide complexes [Ag(GlyGly)]n(NO3)n (AgGlyGly), [Ag2(GlyAla)(NO3)2]n (AgGlyAla) and [Ag2(HGlyAsp)(NO3)]n (AgGlyAsp) were prepared, investigated and characterized by vibrational spectroscopy (mid-IR), elemental and thermogravimetric analysis and mass spectrometry. For AgGlyGly, X-ray crystallography was also performed. Their stability in biological testing media was verified by time-dependent NMR measurements. Their in vitro antimicrobial activity was evaluated against selected pathogenic microorganisms. Moreover, the influence of silver(I) dipeptide complexes on microbial film formation was described. Further, the cytotoxicity of the complexes against selected cancer cells (BLM, MDA-MB-231, HeLa, HCT116, MCF-7 and Jurkat) and fibroblasts (BJ-5ta) using a colorimetric MTS assay was tested, and the selectivity index (SI) was identified. The mechanism of action of Ag(I) dipeptide complexes was elucidated and discussed by the study in terms of their binding affinity toward the CT DNA, the ability to cleave the DNA and the ability to influence numbers of cells within each cell cycle phase. The new silver(I) dipeptide complexes are able to bind into DNA by noncovalent interaction, and the topoisomerase I inhibition study showed that the studied complexes inhibit its activity at a concentration of 15 μM.
Databáze: OpenAIRE
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