Validation of the diagnostic and prognostic relevance of serum MMP-7 levels in renal cell cancer by using a novel automated fluorescent immunoassay method
| Autor: | Miklós Szucs, Frank vom Dorp, Stephan Tschirdewahn, Christian Niedworok, Tibor Szarvas, Henning Reis, Herbert Rübben |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Nephrology Male medicine.medical_specialty Pathology Time Factors Urology Concordance Medizin Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Matrix metalloproteinase Gastroenterology Metastasis 03 medical and health sciences Automation 0302 clinical medicine Internal medicine Biomarkers Tumor Medicine Humans Elisa method Carcinoma Renal Cell Aged Retrospective Studies business.industry Retrospective cohort study medicine.disease Prognosis Kidney Neoplasms 030104 developmental biology 030220 oncology & carcinogenesis FLUORESCENT IMMUNOASSAY Matrix Metalloproteinase 7 Disease Progression Cell cancer Female business Follow-Up Studies |
| Zdroj: | International urology and nephrology. 48(3) |
| ISSN: | 1573-2584 |
| Popis: | Despite encouraging results in other cancers, in renal cell cancer, no consensus exists regarding the diagnostic and prognostic relevance of MMP-7. The aim of this study was to assess the diagnostic and prognostic potential of serum MMP-7 levels in renal cell cancer. Furthermore, parallel to the widely used ELISA method, we tested a new, fluid-phase, fluorescent immunoassay (B.R.A.H.M.S KRYPTOR®) for the quantitation of MMP-7. We analyzed the serum samples of 174 individuals (77 patients and 97 age-matched healthy controls) by a commercially available sandwich ELISA and by a novel, automated, fluid-phase immunofluorescent assay (B.R.A.H.M.S KRYPTOR®). Results were correlated with the clinicopathological and follow-up data. MMP-7 concentrations showed a high concordance level (R 2 = 0.979) between the two methods (p |
| Databáze: | OpenAIRE |
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