Quantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursa

Autor: Maria Margarida Santos-Silva, Margarita Villar, Gustavo Seron Sanches, Olga Moreira, Ana Sofia Santos, José de la Fuente, Lourdes Mateos-Hernández, Joao P. Nobre, Joana Ferrolho, Ana Domingos, Joana Couto, Sandra Antunes
Přispěvatelé: Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Global Health and Tropical Medicine [Lisbonne, Portugal] (GHTM), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana [Espagne] (FISABIO), Biochemistry Section, Faculty of Science and Chemical Technologies, Regional Centre for Biomedical Research ((CRIB)), University of Castilla-La Mancha (UCLM)-University of Castilla-La Mancha (UCLM)-Regional Centre for Biomedical Research ((CRIB)), University of Castilla-La Mancha (UCLM)-University of Castilla-La Mancha (UCLM), Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Instituto de Saúde Ambiental, Faculdade de Medicina, Universidade de Lisboa (ULISBOA)-Universidade de Lisboa (ULISBOA), Instituto Nacional de Investigação Agraria e Veterinaria, Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University [Stillwater]-Oklahoma State University [Stillwater], Fundacao para a Ciencia e Tecnologia (FCT) under the project TickOmic PTDC/CVT-CVT/29073/2017Portuguese Foundation for Science and TechnologySFRH/BD/121946/2016, Fundação para a Ciência e a Tecnologia (Portugal), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Vaccines, Vol 8, Iss 1, p 91 (2020)
Vaccines
Vaccines, MDPI, 2020, 8 (1), pp.91. ⟨10.3390/vaccines8010091⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
Volume 8
Issue 1
ISSN: 2076-393X
DOI: 10.3390/vaccines8010091⟩
Popis: The negative impact of ticks and tick-borne diseases on animals and human health is driving research to discover novel targets affecting both vectors and pathogens. The salivary glands are involved in feeding and pathogen transmission, thus are considered as a compelling target to focus research. In this study, proteomics approach was used to characterize Rhipicephalus bursa sialoproteome in response to Babesia ovis infection and blood feeding. Two potential tick protective antigens were identified and its influence in tick biological parameters and pathogen infection was evaluated. Results demonstrate that the R. bursa sialoproteome is highly affected by feeding but infection is well tolerated by tick cells. The combination of both stimuli shifts the previous scenario and a more evident pathogen manipulation can be suggested. Knockdown of ub2n led to a significative increase of infection in tick salivary glands but a brusque decrease in the progeny, revealing its importance in the cellular response to pathogen infection, which is worth pursuing in future studies. Additionally, an impact in the recovery rate of adults (62%), the egg production efficiency (45.75%), and the hatching rate (88.57 %) was detected. Building knowledge on vector and/or pathogen interplay bridges the identification of protective antigens and the development of novel control strategies.
This research was funded by Fundação para a Ciência e Tecnologia (FCT) under the project TickOmic (PTDC/CVT-CVT/29073/2017). JC and JF are the recipients of Ph.D. grants supported by the FCT (SFRH/BD/121946/2016, SFRH/BD/122894/2016, respectively).
Databáze: OpenAIRE
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