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Results are reported from an experiment designed to test the feasibility of measuring part of the overall genetic damage of irradiation by means of skeletal studies in the F/sub 1/ generation. Male 101-strain mice were irradiated, using different radiation sources and conditions, and subsequently the males were mated to adult C3H-strain femaies. Progeny was selected to sample gametes irradiated in different stages of spermatogenesis. The F/sub 1/ generation was sacrificed at 26 to 28 days of age and animals were processed for skeletal study by a modification of Dawson's technique. Observed abnormalities were classified according to whether they occurred only once in the whole experiment (Class 1) or more than once (Class 2). For Class-1 abnormalities, a highiy significant increase in frequency was observed in offspring of irradiated males. This increase is due largely, if not wholly, to irradiation in postspermatogonial stages. For Class-2 abnormalities, no differences between irradiated and control groups couid be detected. Therefore, we conclude that the classification used served its original purpose of distinguishing between the effects of newly occurring dominant genetic changes and variability from environmental causes. With the methods used, studies of skeletal abnormalities in the first generation can detect part of the overallmore » genetic damage from irradiation. (auth)« less |
