Lack of reliability of nanotechnology in the of free plasma DNA in samples of patients with prostate cancer
| Autor: | Ligia Ajaime Azzalis, Viviane Av Boas, Virginia Bc Junqueira, Jefferson Drezzet, Katya Cristina Rocha, Luiz Carlos de Abreu, Sarah R Marsicano, Edimar Cristiano Pereira, Pamela de Oliveira Delgado, Ricardo Moreno, Fernando La Fonseca, Patricia Granja Coelho, Vitor Engrácia Valenti |
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| Přispěvatelé: | Faculdade de Medicina do ABC (FMABC), Universidade Federal de São Paulo (UNIFESP), Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Blood level
cancer patient Short Report Nanotechnology DNA determination blood level Prostate cancer male Prostate Maximum difference Medicine spectrophotometry human DNA extraction centrifugation reliability nanotechnology business.industry Plasma dna General Medicine DNA medicine.disease prostate cancer major clinical study medicine.anatomical_structure Neoplams Medicine public health blood sampling business Blood sampling |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP International Archives of Medicine |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:28:10Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:37:13Z : No. of bitstreams: 1 2-s2.0-84872131878.pdf: 264619 bytes, checksum: f1b166deaa25d9ab41c2fc74bbd08163 (MD5) Made available in DSpace on 2014-05-27T11:28:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-01-16 Background: Several studies seek biological markers that give diagnostic and degree of tumor development. The aim of this study was to validate the determination of plasma DNA using nanotechnology (Nanovue™-NV) in samples of 80 patients with prostate cancer. Methods. Blood samples of 80 patients of the Urology Ambulatory of Faculdade de Medicina do ABC with prostate cancer confirmed by anatomical-pathology criteria were analyzed. DNA extraction was performed using a GFX TM kit (Amersham Pharmacia Biotech, Inc, USA) following the adapted protocol. Plasma was subjected to centrifugation. Results: There was a big difference between the first and the second value obtained by NanoVue Only two samples had no differences between duplicates. Maximum difference between duplicates was 38 μg/mL. Average variation between 51 samples was 10.29 μg/mL, although 21 samples had differences above this average. No correlation was observed between pDNA obtained by traditional spectrophotometry and by nanotechnology. Conclusion: Determination of plasma DNA by nanotechnology was not reproducible. © 2013 Moreno et al; licensee BioMed Central Ltd. Laboratório de Análises Clínicas Faculdade de Medicina Do ABC, Av. Príncipe de Gales 821, Santo André, CEP: 09060-650 Departamento de Ciências Biológicas Universidade Federal de São Paulo UNIFESP, Rua Prof. Artur Riedel, 275, Diadema, São Paulo 09972-270 Laboratório de Delineamento de Estudos e Escrita Científica Departamento de Morfologia e Fisiologia Faculdade de Medicina Do ABC, Santo André, Av. Príncipe de Gales, n. 821, Santo André, CEP: 09060-650 Programa de Pós-Graduação em Fisioterapia Faculdade de Ciências e Tecnologia Universidade Estadual Paulista, Rua Roberto Simonsen, 305, Presidente Prudente, São Paulo 19060-900 Programa de Pós-Graduação em Fisioterapia Faculdade de Ciências e Tecnologia Universidade Estadual Paulista, Rua Roberto Simonsen, 305, Presidente Prudente, São Paulo 19060-900 |
| Databáze: | OpenAIRE |
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