Quantal acetylcholine release induced by mediatophore transfection
| Autor: | E. Roulet, Françoise Loctin, Alain Bloc, M. Synguelakis, B. Lesbats, Yves Dunant, L Eder-Colli, Maurice Israël, J Falk-Vairant, N Salem, François-Marie Meunier, P Corrèges |
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| Rok vydání: | 1996 |
| Předmět: |
Macromolecular Substances
Protein subunit Gene Expression chemistry.chemical_element Nerve Tissue Proteins Calcium Biology Torpedo Transfection Cell Line law.invention Neuroblastoma law Gene expression Tumor Cells Cultured medicine Animals Multidisciplinary Acetylcholine Recombinant Proteins Clone Cells Cell biology Kinetics chemistry Cell culture Cholinergic Research Article medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:5203-5207 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.93.11.5203 |
| Popis: | Mediatophore is a protein of approximately 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subunit, homologous to the proteolipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to produce quantal acetylcholine release. We show here that transfection of N18TG-2 cells with a plasmid encoding the mediatophore subunit restored calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed in situ in cholinergic synapses from which mediatophore was purified. |
| Databáze: | OpenAIRE |
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