Effect of IL-34 on T helper 17 cell proliferation and IL-17 secretion by peripheral blood mononuclear cells from rheumatoid arthritis patients
Autor: | Ziyu Gao, Bei Zhang, Liping Xia, Yimeng Lei, Jing Lu, Xin Li, Hui Shen |
---|---|
Rok vydání: | 2020 |
Předmět: |
Male
0301 basic medicine Science medicine.medical_treatment Cell Communication Lymphocyte Activation Peripheral blood mononuclear cell Article Immunophenotyping Flow cytometry Arthritis Rheumatoid 03 medical and health sciences 0302 clinical medicine T-Lymphocyte Subsets Humans Medicine T helper 17 cell Secretion Rheumatoid arthritis Cells Cultured Aged 030203 arthritis & rheumatology Multidisciplinary medicine.diagnostic_test business.industry Interleukins Interleukin-17 Interleukin Middle Aged 030104 developmental biology Cytokine Gene Expression Regulation Preclinical research Immunology Leukocytes Mononuclear Cytokines Th17 Cells Female Cytokine secretion Interleukin 17 business Biomarkers |
Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) |
ISSN: | 2045-2322 |
Popis: | Interleukin (IL)-34 is a new pro-inflammatory cytokine with elevated expression in rheumatoid arthritis (RA) patients. Our previous study showed that the frequency of T helper 17 (Th17) cells was also elevated in RA patients. Our study aimed to determine the effects of IL-34 on the proliferation, transcription factor expression and cytokine secretion of different subgroups of CD4 + T cells [Th1, Th2, Th17 and regulatory T (Treg) cells] in RA patients. Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of 10 RA patients and stimulated with different concentrations of recombinant human (rh) IL-34 (0, 25, 50 and 100 ng/ml). Flow cytometry was used to determine the frequencies of the 4 subgroups of CD4 + T cells. Reverse transcription-PCR, western blotting and enzyme-linked immunosorbent assays were used to determine the mRNA and protein expression levels of transcription factors and cytokines. As a result, the frequency of Th17 cells was obviously increased under IL-34 stimulation. Moreover, the expression of the transcription factor retinoic acid-related orphan receptor (ROR-γt) and secretion of IL-17 by PBMCs were increased by stimulation with IL-34. However, there were no effects of IL-34 on transcription factors or cytokine secretion in Th1, Th2 and Treg cells. In conclusion, IL-34 can improve the proliferation of Th17 cells and expression of IL-17 in RA patients. |
Databáze: | OpenAIRE |
Externí odkaz: |