(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes
| Autor: | Chongyang Ge, Mengling Peng, Haitian Ma, Longlong Li, Lei Yu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology Primary chicken hepatocytes Malate dehydrogenase lcsh:Physiology lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound (-)-Hydroxycitric acid 0302 clinical medicine ATP-citrate Acetyl Coenzyme A Lipid droplet Citrate synthase Animals lcsh:QD415-436 Citrates Lyase activity Cells Cultured lcsh:QP1-981 biology Acetyl-CoA Lipid metabolism Lipid Droplets Pyruvate dehydrogenase complex Lipid Metabolism Fatty acid synthase 030104 developmental biology Biochemistry chemistry 030220 oncology & carcinogenesis biology.protein Hepatocytes Energy Metabolism Chickens |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 43, Iss 2, Pp 812-831 (2017) |
| ISSN: | 1421-9778 |
| Popis: | Background/Aims: (-)-Hydroxycitric acid (HCA) had been shown to suppress fat accumulation in animals and humans, while the underlying biochemical mechanism is not fully understood, especially little information is available on whether (-)-HCA regulates energy metabolism and consequently affects fat deposition. Methods: Hepatocytes were cultured for 24 h and then exposed to (-)-HCA (0, 1, 10, 50 µM), enzyme protein content was determined by ELISA; lipid metabolism gene mRNA levels were detected by RT-PCR. Results: (-)-HCA significantly decreased the number and total area of lipid droplets. ATP-citrate lyase, fatty acid synthase and sterol regulatory element binding protein-1c mRNA level were significantly decreased after (-)-HCA treatment, whereas peroxisome proliferator-activated receptor α mRNA level was significantly increased. (-)-HCA significantly decreased ATP-citrate lyase activity and acetyl-CoA content in cytosol, but significantly increased glucose consumption and mitochondrial oxygen consumption rate. (-)-HCA promoted the activity/content of glucokinase, phosphofructokinase-1, pyruvate kinase, pyruvate dehydrogenase, citrate synthase, aconitase, succinate dehydrogenase, malate dehydrogenase, NADH dehydrogenase and ATP synthase remarkably. Conclusions: (-)-HCA decreased lipid droplets accumulation by reducing acetyl-CoA supply, which mainly achieved via inhibition of ATP-citrate lyase, and accelerating energy metabolism in chicken hepatocytes. These results proposed a biochemical mechanism of fat reduction by (-)-HCA in broiler chickens in term of energy metabolism. |
| Databáze: | OpenAIRE |
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