Mitochondrial ribosomal proteins involved in tellurite resistance in yeast Saccharomyces cerevisiae
| Autor: | Jacopo Troisi, Paola Pontieri, Hans Hartings, Marco Di Salvo, Mario De Stefano, Roberta Romano, Pietro Alifano, Angelica Del Giudice, Domenica Rita Massardo, Graziano Pizzolante, Luigi Del Giudice |
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| Přispěvatelé: | Pontieri, Paola, Hartings, Han, Di Salvo, Marco, Massardo, Domenica R., De Stefano, Mario, Pizzolante, Graziano, Romano, Roberta, Troisi, Jacopo, Del Giudice, Angelica, Alifano, Pietro, Del Giudice, Luigi, Massardo, Domenica R |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Ribosomal Proteins
0301 basic medicine Saccharomyces cerevisiae Proteins genetic structures 030106 microbiology Mutant Saccharomyces cerevisiae lcsh:Medicine Biology medicine.disease_cause Article Mitochondrial Proteins Transcriptome 03 medical and health sciences Microscopy Electron Transmission Ribosomal protein Gene Expression Regulation Fungal Mitochondrial ribosome medicine Saccharomyces cerevisiae Mitochondrial ribosomal proteins Tellurite resistance lcsh:Science Gene Genetics Mutation Multidisciplinary Gene Expression Profiling lcsh:R xxx Ribosomal RNA biology.organism_classification Mitochondria lcsh:Q Tellurium Ribosomes |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Scientific Reports Scientific reports (Nature Publishing Group) 8 (2018). doi:10.1038/s41598-018-30479-6 info:cnr-pdr/source/autori:Pontieri P.; Hartings H.; Di Salvo M.; Massardo D.R.; De Stefano M.; Pizzolante G.; Romano R.; Troisi J.; Del Giudice A.; Alifano P.; Del Giudice L./titolo:Mitochondrial ribosomal proteins involved in tellurite resistance in yeast Saccharomyces cerevisiae/doi:10.1038%2Fs41598-018-30479-6/rivista:Scientific reports (Nature Publishing Group)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:8 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-30479-6 |
| Popis: | A considerable body of evidence links together mitochondrial dysfunctions, toxic action of metalloid oxyanions, and system and neurodegenerative disorders. In this study we have used the model yeast Saccharomyces cerevisiae to investigate the genetic determinants associated with tellurite resistance/sensitivity. Nitrosoguanidine-induced K2TeO3-resistant mutants were isolated, and one of these mutants, named Sc57-Te5R, was characterized. Both random spore analysis and tetrad analysis and growth of heterozygous (TeS/Te5R) diploid from Sc57-Te5R mutant revealed that nuclear and recessive mutation(s) was responsible for the resistance. To get insight into the mechanisms responsible for K2TeO3-resistance, RNA microarray analyses were performed with K2TeO3-treated and untreated Sc57-Te5R cells. A total of 372 differentially expressed loci were identified corresponding to 6.37% of the S. cerevisiae transcriptome. Of these, 288 transcripts were up-regulated upon K2TeO3 treatment. About half of up-regulated transcripts were associated with the following molecular functions: oxidoreductase activity, structural constituent of cell wall, transporter activity. Comparative whole-genome sequencing allowed us to identify nucleotide variants distinguishing Sc57-Te5R from parental strain Sc57. We detected 15 CDS-inactivating mutations, and found that 3 of them affected genes coding mitochondrial ribosomal proteins (MRPL44 and NAM9) and mitochondrial ribosomal biogenesis (GEP3) pointing out to alteration of mitochondrial ribosome as main determinant of tellurite resistance. |
| Databáze: | OpenAIRE |
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