Short-term cold storage of seminal samples of the golden lancehead pitviper (Bothrops insularis)
| Autor: | Mayra Hespanhol Frediani, Ricardo José Garcia Pereira, Daniel de Souza Ramos Angrimani, Kalena Barros da Silva, Marcilio Nichi, Selma Maria Almeida-Santos, Rafaela Zani Coeti |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0106 biological sciences endocrine system Time Factors Bothrops insularis medicine.medical_treatment Cold storage Zoology Semen Reproductive technology 010603 evolutionary biology 01 natural sciences Cryopreservation law.invention law medicine Animals Bothrops 0501 psychology and cognitive sciences 050102 behavioral science & comparative psychology biology Artificial insemination 05 social sciences Extender General Medicine biology.organism_classification Sperm Cold Temperature Semen Analysis Animal Science and Zoology Semen Preservation PRESERVAÇÃO DO SÊMEN ANIMAL |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Assisted reproductive technologies such as cooling, freezing, and artificial insemination are essential in conserving genetic diversity of critically endangered reptiles like the golden lancehead pitviper (Bothrops insularis). Therefore, we examined viability of semen samples from captive golden lanceheads diluted in coconut-water based extender over a 48-h period of cooling (5°C). Semen evaluations were performed immediately after the dilution and at 6, 24, and 48 h, using computer-assisted sperm analysis and stains to assess plasma membrane and acrosomal status. Our findings showed that the extender and protocol employed here were effective in preserving golden lancehead pitviper spermatozoa for a short periods of time (48 h), allowing semen samples to be transported to distant locations for immediate use without the setbacks of cryopreservation. |
| Databáze: | OpenAIRE |
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