Antioxidant Effect of Ukrain VersusN-Acetylcysteine Against Acute Biliary Pancreatitis in An Experimental Rat Model
| Autor: | Mustafa Cem Algin, Ahmet Kocak, Cengiz Koçak, Bircan Savran, Sezgin Zeren, Havva Koçak, Fatma Emel Koçak, Süleyman Cosgun, Zülfü Bayhan, Cüneyt Kahraman, Mehmet Hüseyin Metineren |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
Antioxidant medicine.medical_treatment Berberine Alkaloids Pharmacology medicine.disease_cause Antioxidants Rats Sprague-Dawley Pathogenesis Acetylcysteine 0302 clinical medicine Malondialdehyde Biliary Tract Alanine Transaminase Oxidants Phenanthridines 030220 oncology & carcinogenesis Amylases Acute pancreatitis 030211 gastroenterology & hepatology medicine.drug medicine.medical_specialty Rat model Nitric Oxide 03 medical and health sciences Internal medicine medicine Animals Humans Aspartate Aminotransferases Pancreas Peroxidase biliary system Tumor Necrosis Factor-alpha business.industry Therapeutic effect Bilirubin Lipase medicine.disease Ukrain Rats Disease Models Animal Oxidative Stress Endocrinology Pancreatitis nervous system Surgery business Oxidative stress |
| Zdroj: | Journal of Investigative Surgery. 30:116-124 |
| ISSN: | 1521-0553 0894-1939 |
| Popis: | Purpose/Aim: Oxidative stress plays an important role in the pathogenesis of acute pancreatitis (AP). We compared the therapeutic effects of Ukrain (NSC 631570) and N-acetylcysteine (NAC) in rats with AP. Materials and Methods: Forty male Sprague Dawley rats were divided into four groups: controls; AP; AP with NAC; and AP with Ukrain. AP was induced via the ligation of the bile–pancreatic duct; drugs were administered intraperitoneally (i.p.) 30 min and 12 h after AP induction. Twenty-four hours after AP induction, animals were sacrificed and the pancreas was excised. Levels of malondialdehyde (MDA) and nitric oxide (NO), and activity levels of tumor necrosis factor (TNF)-?, and myeloperoxidase (MPO) were measured in tissue samples. Total oxidant status (TOS), total antioxidant status (TAS), and total bilirubin, as well as activity levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), amylase and lipase were measured in serum samples. Pancreatic tissue histopathology was also evaluated. Results: Test drugs reduced levels of MDA, NO, TNF-?, total bilirubin, AST, ALT, TOS and MPO, amylase and lipase activities (P < 0.001), and increased TAS (P < 0.001). Rats treated with test drugs attenuated AP-induced morphologic changes and decreased pancreatic damage scores compared with the AP group (P < 0.05). Both test drugs attenuated pancreatic damage, but the therapeutic effect was more pronounced in rats that received Ukrain than in those receiving NAC. Conclusions: These results suggest that treatment with Ukrain or NAC can reduce pancreatic damage via anti-inflammatory and antioxidant effects. Copyright © 2017 Taylor & Francis Group, LLC. |
| Databáze: | OpenAIRE |
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