Generation of two induced pluripotent stem cell lines (RCMGi005-A/B) from human skin fibroblasts of a cystic fibrosis patient with homozygous F508del mutation in CFTR gene
Autor: | Irina Panchuk, Ekaterina Kondrateva, Anna Demchenko, Olga Grigorieva, Anastasija Erofeeva, Elena Amelina, Vyacheslav Tabakov, Maria Orlova, Ekaterina Voronina, Victoria Pozhitnova, Alexander Lavrov, Svetlana Smirnikhina, Sergey Kutsev |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Stem cell research. 64 |
ISSN: | 1876-7753 |
Popis: | Induced pluripotent stem cells (iPSCs) was successfully generated from skin fibroblast obtained from patient with cystic fibrosis by using non-integrating, viral CytoTune™-iPS 2.0 Sendai Reprogramming Kit, which contain three vectors preparation: polycistronic Klf4-Oct3/4-Sox2, cMyc, and Klf4. Created iPSC lines showed a normal karyotype, expressed pluripotency markers and demonstrated the potential to differentiate into three germ layers in spontaneous differentiation assay. |
Databáze: | OpenAIRE |
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