Generation of two induced pluripotent stem cell lines (RCMGi005-A/B) from human skin fibroblasts of a cystic fibrosis patient with homozygous F508del mutation in CFTR gene

Autor:	Irina Panchuk, Ekaterina Kondrateva, Anna Demchenko, Olga Grigorieva, Anastasija Erofeeva, Elena Amelina, Vyacheslav Tabakov, Maria Orlova, Ekaterina Voronina, Victoria Pozhitnova, Alexander Lavrov, Svetlana Smirnikhina, Sergey Kutsev
Rok vydání:	2022
Předmět:	Inducible T-Cell Co-Stimulator Protein Cystic Fibrosis Induced Pluripotent Stem Cells Mutation Humans Cystic Fibrosis Transmembrane Conductance Regulator Cell Differentiation Cell Biology General Medicine Fibroblasts Developmental Biology
Zdroj:	Stem cell research. 64
ISSN:	1876-7753
Popis:	Induced pluripotent stem cells (iPSCs) was successfully generated from skin fibroblast obtained from patient with cystic fibrosis by using non-integrating, viral CytoTune™-iPS 2.0 Sendai Reprogramming Kit, which contain three vectors preparation: polycistronic Klf4-Oct3/4-Sox2, cMyc, and Klf4. Created iPSC lines showed a normal karyotype, expressed pluripotency markers and demonstrated the potential to differentiate into three germ layers in spontaneous differentiation assay.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e4603d5f2934eeb4ef786444d6e2666 https://pubmed.ncbi.nlm.nih.gov/36067639 Zobrazit plný text záznamu