Diagnosis of congenital Trypanosoma cruzi infection: A serologic test using Shed Acute Phase Antigen (SAPA) in mother-child binomial samples
| Autor: | Bibiana Julieta Volta, Zunilda Sánchez, Rita L. Cardoni, Graciela Russomando, Ana María de Rissio, Karenina Scollo, Jacqueline Bua, Patricia Laura Bustos |
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| Rok vydání: | 2014 |
| Předmět: |
INFANTS
Antibodies Protozoan SHED ACUTE PHASE ANTIGEN Parasitemia Parasite Load Serology Pregnancy CONGENITAL INFECTION Antibody titer SAPA Infectious Diseases Female Antibody CIENCIAS NATURALES Y EXACTAS Chagas disease Adult Veterinary (miscellaneous) Otras Ciencias Biológicas Trypanosoma cruzi Mothers Neuraminidase Enzyme-Linked Immunosorbent Assay Biology Ciencias Biológicas Young Adult Antigen parasitic diseases medicine Humans Chagas Disease Serologic Tests Glycoproteins Retrospective Studies SEROLOGY Infant Newborn Infant TRYPANOSOMA CRUZI medicine.disease biology.organism_classification Virology Infectious Disease Transmission Vertical Early Diagnosis Insect Science Pregnancy Complications Parasitic Immunology biology.protein Parasitology PREGNANT WOMEN |
| Zdroj: | Acta tropica. 147 |
| ISSN: | 1873-6254 |
| Popis: | Chagas congenital infection is an important health problem in endemic and non-endemic areas in which Trypanosoma cruzi-infected women can transmit the parasite to their offspring. In this study, we evaluated the antibody levels against the T. cruzi Shed Acute Phase Antigen (SAPA) in 91 binomial samples of seropositive pregnant women and their infected and non-infected children by ELISA. In 70 children without congenital T. cruzi transmission, the titers of anti-SAPA antibodies were lower than those of their seropositive mothers. In contrast, 90.5% of 21 congenitally infected children, at around 1 month of age, showed higher anti-SAPA antibody levels than their mothers. Subtracting the SAPA-ELISA mother OD value to the SAPA-ELISA child OD allowed efficient detection of most T. cruzi congenitally infected children immediately after birth, when total anti-parasite antibodies transferred during pregnancy are still present in all children born to seropositive women. A positive correlation was observed between parasitemia levels in mothers and infants evaluated by quantitative DNA amplification and anti-SAPA antibody titers by ELISA. As SAPA serology has proved to be very efficient to detect T. cruzi infection in mother-child binomial samples, it could be of extreme help for early diagnosis of newborns, in maternities and hospitals where DNA amplification is not available. This prompt diagnosis may prevent drop out of the long-term follow-up for future diagnosis and may ensure early trypanocidal treatment, which has proved to be efficient to cure infants with congenital Chagas disease. Fil: Volta, Bibiana Julieta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina Fil: Russomando, Graciela Mabel. Universidad Nacional de Asunción; Paraguay Fil: Bustos, Patricia Laura. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scollo, Karenina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina Fil: de Rissio, Ana María. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina Fil: Sánchez, Zunilda. Universidad Nacional de Asunción; Paraguay Fil: Cardoni, Rita Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina Fil: Bua, Jacqueline Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina. Universidad Abierta Interamericana; Argentina |
| Databáze: | OpenAIRE |
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