Influence of Specific Bacteriophage on the Level of Vesicle Formation and Morphology of Cells of Yersinia pseudotuberculosis
| Autor: | E. A. Martinson, A. V. Chernyad’ev, S. G. Litvinets, M. A. Malkova, L. G. Dudina, A. A. Byvalov |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Morphology (linguistics) biology Chemistry Vesicle 030106 microbiology General Medicine biology.organism_classification Solid medium General Biochemistry Genetics and Molecular Biology Suspension (chemistry) Microbiology Culture Media Bacteriophage 03 medical and health sciences Extracellular Vesicles Microscopy Electron 030104 developmental biology Yersinia pseudotuberculosis Microbial Interactions Bacteriophages Incubation Bacteria |
| Zdroj: | Bulletin of experimental biology and medicine. 165(3) |
| ISSN: | 1573-8221 |
| Popis: | Incubation of Yersinia pseudotuberculosis cells grown on a solid medium with pseudotuberculous diagnostic bacteriophage for 20 min at 37oC led to a significant decrease in the concentration of both components of the system. This effect was absent when the bacteria were grown in a fluid medium. At the same time, this incubation regimen promoted vesicle formation and typical morphological changes in bacteria grown in both surface and suspension cultures. Co-incubation of the bacteriophage with suspension of vesicles isolated from the suspension culture of Y. pseudotuberculosis grown at 10oC (but not 37oC) led to a decrease in plaque-forming activity of the bacteriophage. |
| Databáze: | OpenAIRE |
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