DNA fingerprinting--a valuable new technique for the characterisation of cell lines
| Autor: | Bryan Griffiths, Glyn Stacey, Alan Doyle, B. J. Bolton |
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| Rok vydání: | 1992 |
| Předmět: |
Genetics
Mouse strain Cellular differentiation Clinical Biochemistry Biomedical Engineering Chromosome Mapping Reproducibility of Results Bioengineering Cell Differentiation Cell Biology Computational biology Biology DNA Fingerprinting Cell Line DNA profiling Cell culture Fish Animals Humans Human genome Biotechnology |
| Zdroj: | Cytotechnology. 9(1-3) |
| ISSN: | 0920-9069 |
| Popis: | DNA fingerprinting is an important new development for the authentication of cell lines. Multilocus methods such as those developed by Alec Jeffreys provide information on a wide range of genetic loci throughout the human genome and thus give a useful genetic "snap-shot" of a cell culture. Our work has shown that Jeffreys multilocus fingerprinting method can be applied to cell lines from a wide range of animals including reptiles, birds, fish and diverse mammals. It can also differentiate very closely related cell lines including those from the same mouse strain. Routine fingerprint analysis has enabled an unprecedented level of confidence in the consistency of cell stocks. Our results demonstrate that this straightforward method represents a powerful and readily interpreted system for cell authentication and exclusion of cross-contamination. |
| Databáze: | OpenAIRE |
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