Population Dynamics of Staphylococcus aureus in Cystic Fibrosis Patients To Determine Transmission Events by Use of Whole-Genome Sequencing
| Autor: | Andrea Ankrum, Barry G. Hall |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Staphylococcus aureus Adolescent Cystic Fibrosis Epidemiology Population Dynamics Population Single-nucleotide polymorphism Biology Staphylococcal infections medicine.disease_cause Polymorphism Single Nucleotide Microbiology Young Adult 03 medical and health sciences Genetic variation medicine Humans SNP Child education Phylogeny Whole genome sequencing education.field_of_study Whole Genome Sequencing Phylogenetic tree Infant Newborn Genetic Variation Infant Staphylococcal Infections medicine.disease 030104 developmental biology Child Preschool Female |
| Zdroj: | Journal of Clinical Microbiology. 55:2143-2152 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00164-17 |
| Popis: | Strict infection control practices have been implemented for health care visits by cystic fibrosis (CF) patients in an attempt to prevent transmission of important pathogens. This study used whole-genome sequencing (WGS) to determine strain relatedness and assess population dynamics of Staphylococcus aureus isolates from a cohort of CF patients as assessed by strain relatedness. A total of 311 S. aureus isolates were collected from respiratory cultures of 115 CF patients during a 22-month study period. Whole-genome sequencing was performed, and using single nucleotide polymorphism (SNP) analysis, phylogenetic trees were assembled to determine relatedness between isolates. Methicillin-resistant Staphylococcus aureus (MRSA) phenotypes were predicted using PPFS2 and compared to the observed phenotype. The accumulation of SNPs in multiple isolates obtained over time from the same patient was examined to determine if a genomic molecular clock could be calculated. Pairs of isolates with ≤71 SNP differences were considered to be the “same” strain. All of the “same” strain isolates were either from the same patient or siblings pairs. There were 47 examples of patients being superinfected with an unrelated strain. The predicted MRSA phenotype was accurate in all but three isolates. Mutation rates were unable to be determined because the branching order in the phylogenetic tree was inconsistent with the order of isolation. The observation that transmissions were identified between sibling patients shows that WGS is an effective tool for determining transmission between patients. The observation that transmission only occurred between siblings suggests that Staphylococcus aureus acquisition in our CF population occurred outside the hospital environment and indicates that current infection prevention efforts appear effective. |
| Databáze: | OpenAIRE |
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