Endophytic biocatalysts with enoate reductase activity isolated from Mentha pulegium
| Autor: | Paula Rodríguez, David Gonzalez, Facundo Marconi, Sonia Rodríguez Giordano, María Laura Umpiérrez |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Stereochemistry Bacillus Cyclohexane Monoterpenes 01 natural sciences Applied Microbiology and Biotechnology Kinetic resolution 03 medical and health sciences chemistry.chemical_compound Biotransformation Mentha pulegium Pseudomonas RNA Ribosomal 16S Endophytes Phylogeny Carvone biology Bacteria 010405 organic chemistry General Medicine biology.organism_classification 0104 chemical sciences Plant Leaves Kinetics 030104 developmental biology chemistry Pseudomonas proteolytica Monoterpenes Racemic mixture Oxidoreductases Oxidation-Reduction Pennyroyal Biotechnology |
| Zdroj: | World journal of microbiologybiotechnology. 34(4) |
| ISSN: | 1573-0972 |
| Popis: | The biotransformation of (4R)-(−)-carvone by Mentha pulegium (pennyroyal) leaves and its endophytic bacteria was performed in order to search for novel biocatalysts with enoate reductase activity. The obtained results clearly indicated that endophytes play an important role in the biotransformation of (4R)-(−)-carvone with pennyroyal plant tissues. The best activity was associated to the endophytic bacteria Pseudomonas proteolytica FM18Mci1 and Bacillus sp. FM18civ1. Enoate reductase activity for the reduction of (4R)-(−)-carvone and (4S)-(+)-carvone as model substrates was evaluated for each strain. Finally, both isolated strains were evaluated for the kinetic resolution of racemic carvone. The two bacteria gave (1R, 4R) or (1R, 4S)-dihydrocarvone as major products. P. proteolytica FM18Mci1 had preference for the 4S-(−)-carvone, reaching a conversion 95% in 24 h. In contrast, Bacillus sp. FM18civ1 had preference for (4R)-(−)-carvone. The results obtained in the kinetic resolution of carvone indicated that the Bacillus strain could be useful for resolving a racemic mixture of carvone. |
| Databáze: | OpenAIRE |
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