A novel CEA-cross-reacting antigen of molecular weight 140,000 expressed on human lymphoid cell lines
| Autor: | Kuroki Masahide, Kuroki Motomu, Matsuo Yoshino, Mlnowada Jun, Ohtani Takeshi, Matsuoka Yuji |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.drug_class
T cell Immunology Biology Cross Reactions Immunofluorescence Monoclonal antibody Epitope Cell Line Carcinoembryonic antigen Antigen medicine Humans Lymphocytes Molecular Biology B cell Membrane Glycoproteins medicine.diagnostic_test Phosphoric Diester Hydrolases Phosphatidylinositol Diacylglycerol-Lyase Antibodies Monoclonal Molecular biology Precipitin Tests digestive system diseases Carcinoembryonic Antigen Molecular Weight medicine.anatomical_structure Cell culture Multigene Family biology.protein |
| Zdroj: | Molecular immunology. 27(7) |
| ISSN: | 0161-5890 |
| Popis: | To investigate the possible expression of the carcinoembryonic antigen (CEA) gene family products on lymphoid cells, we screened 28 human cell lines derived from malignant lymphoid cells for reactivity with monoclonal antibodies (MAbs) against CEA and nonspecific cross-reacting antigen (NCA), which is one of the CEA gene family members. Six cell lines (four B cell lines and two non-T, non-B cell lines) were found to react, by a membrane immunofluorescence test, with an MAb, F34-187, which recognizes an antigenic determinant shared between CEA and NCA. None of the 15 T cell lines was reactive with any MAbs tested. A glycoprotein antigen isolated with F34-187 from the cell surface showed an apparent molecular mass of ca 140 and 70 kDa in the glycosylated and deglycosylated forms, respectively, and was unreactive with MAbs specific for CEA or NCA, suggesting that the antigen is a new member of the CEA gene family. |
| Databáze: | OpenAIRE |
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