Disseminated tumour cells as a prognostic biomarker in colorectal cancer

Autor: Heidi Rasmussen, Elin Borgen, Jacobsen Hj, Rosales R, Kjersti Flatmark, Jahn M. Nesland, Kjetil Boye, Øystein Fodstad, Johannessen Ho, Ida Rashida Khan Bukholm, B. Sandstad, Hårklau L
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Oncology
Adult
Male
Cancer Research
medicine.medical_specialty
Pathology
Colorectal cancer
colorectal cancer
Kaplan-Meier Estimate
Immunomagnetic separation
Disease-Free Survival
Antigens
Neoplasm
Bone Marrow
Predictive Value of Tests
Internal medicine
medicine
Humans
Prospective Studies
Prospective cohort study
prognostic biomarker
Molecular Diagnostics
Aged
Neoplasm Staging
Aged
80 and over
Analysis of Variance
business.industry
Immunomagnetic Separation
Norway
Cancer
Middle Aged
medicine.disease
Epithelial Cell Adhesion Molecule
Neoplastic Cells
Circulating
Prognosis
Immunohistochemistry
medicine.anatomical_structure
Predictive value of tests
EpCAM
Population study
Biomarker (medicine)
Keratins
Female
Bone marrow
disseminated tumour cells
business
Colorectal Neoplasms
cytokeratin
Cell Adhesion Molecules
Zdroj: British Journal of Cancer
ISSN: 1532-1827
0007-0920
Popis: BACKGROUND: The study was performed to determine detection rate and prognostic relevance of disseminated tumour cells (DTC) in patients receiving curatively intended surgery for colorectal cancer (CRC). METHODS: The study population consisted of 235 patients with CRC prospectively recruited from five hospitals in the Oslo region. Bone marrow (BM) aspirates were collected at the time of surgery and the presence of DTC was determined by two immunological methods; immunomagnetic selection (using an anti-EpCAM antibody) and immunocytochemistry (using a pan-cytokeratin antibody). Associations between the presence of DTC and metastasis-free, disease-specific and overall survival were analysed using univariate and multivariate methods. RESULTS: Disseminated tumour cells were detected in 41 (17%) and 28 (12%) of the 235 examined BM samples by immunomagnetic selection and immunocytochemistry, respectively, with only five samples being positive with both methods. The presence of DTC was associated with adverse outcome (metastasis-free, disease-specific and overall survival) in univariate and multivariate analyses. CONCLUSION: The presence of DTC was associated with adverse prognosis in this cohort of patients curatively resected for CRC, suggesting that DTC detection still holds promise as a biomarker in CRC.
Databáze: OpenAIRE
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