Nanoparticle Wettability Influences Nanoparticle–Phospholipid Interactions
| Autor: | Flavia Damiani, Philip Demokritou, Svetla Stoilova-McPhie, Ramon M. Molina, Thomas C. Donaghey, Jason S. Tresback, Georgios Pyrgiotakis, Joseph D. Brain, Nagarjun V. Konduru |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Lipid Bilayers
Phospholipid Nanoparticle 02 engineering and technology 010402 general chemistry 01 natural sciences Article chemistry.chemical_compound Adsorption Electrochemistry Animals General Materials Science Surface charge Lipid bilayer Spectroscopy Phospholipids Cryoelectron Microscopy technology industry and agriculture Surfaces and Interfaces Phosphatidic acid respiratory system 021001 nanoscience & nanotechnology Condensed Matter Physics Silicon Dioxide 0104 chemical sciences Rats chemistry Chemical engineering Transmission electron microscopy Wettability Nanoparticles lipids (amino acids peptides and proteins) 0210 nano-technology Sphingomyelin |
| Popis: | We explored the influence of nanoparticle (NP) surface charge and hydrophobicity on NP—biomolecule interactions by measuring the composition of adsorbed phospholipids on four NPs, namely, positively charged CeO(2) and ZnO and negatively charged BaSO(4) and silica-coated CeO(2), after exposure to bronchoalveolar lavage fluid (BALf) obtained from rats, and to a mixture of neutral dipalmitoyl phosphatidylcholine (DPPC) and negatively charged dipalmitoyl phosphatidic acid (DPPA). The resulting NP—lipid interactions were examined by cryogenic transmission electron microscopy (cryo-TEM) and atomic force microscopy (AFM). Our data show that the amount of adsorbed lipids on NPs after incubation in BALf and the DPPC/DPPA mixture was higher in CeO(2) than in the other NPs, qualitatively consistent with their relative hydrophobicity. The relative concentrations of specific adsorbed phospholipids on NP surfaces were different from their relative concentrations in the BALf. Sphingomyelin was not detected in the extracted lipids from the NPs despite its >20% concentration in the BALf. AFM showed that the more hydrophobic CeO(2) NPs tended to be located inside lipid vesicles, whereas less hydrophobic BaSO(4) NPs appeared to be outside. In addition, cryo-TEM analysis showed that CeO(2) NPs were associated with the formation of multilamellar lipid bilayers, whereas BaSO(4) NPs with unilamellar lipid bilayers. These data suggest that the NP surface hydrophobicity predominantly controls the amounts and types of lipids adsorbed, as well as the nature of their interaction with phospholipids. |
| Databáze: | OpenAIRE |
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