The Drosophila RNA-binding protein ELAV is required for commissural axon midline crossing via control of commissureless mRNA expression in neurons
| Autor: | Elena Simionato, Natalia Barrios, Elisabeth Boissonneau, Louise Duloquin, François Agnès, Pierrette Lecorre |
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| Přispěvatelé: | Développement et évolution (DE), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Centre de génétique moléculaire (CGM), Centre National de la Recherche Scientifique (CNRS), CBMSO Campus de la Universidad Autónoma de Madrid, Universidad Autónoma de Madrid (UAM), Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Observatoire océanologique de Villefranche-sur-mer (OOVM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud 11, CNRS, European Community, 'La plante et son environnement' is supported by Action de Soutien à la Technologie et la Recherche en Essonne, Conseil de l'Essonne., Universidad Autonoma de Madrid (UAM) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Central Nervous System
RNA-binding protein Mutant Commissure Robo Biology 03 medical and health sciences 0302 clinical medicine Slit medicine Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Axon Growth cone Molecular Biology Body Patterning 030304 developmental biology Neurons Genetics 0303 health sciences Cell Death Axon guidance RNA-Binding Proteins Cell Biology Immunohistochemistry Axons Cell biology medicine.anatomical_structure Hu Neural development Commissureless Midline crossing Drosophila Neuron 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology Developmental Biology, 2007, 301 (1), pp.166-77. ⟨10.1016/j.ydbio.2006.09.028⟩ Developmental Biology, Elsevier, 2007, 301 (1), pp.166-77. ⟨10.1016/j.ydbio.2006.09.028⟩ |
| ISSN: | 0012-1606 1095-564X |
| Popis: | Drosophila ELAV is the founding member of an evolutionarily conserved family of RNA-binding proteins considered as key inducers of neuronal differentiation. Although several ELAV-specific targets have been identified, little is known about the role of elav during neural development. Here, we report a detailed characterization of the elav mutant commissural phenotype. The reduced number of commissures in elav mutant embryos is not due to loss or misspecification of neural cells but results from defects in commissural axon projections across the midline. We establish a causal relationship between the elav mutant commissural phenotype and a reduction in the expression of commissureless, a key component of the Robo/Slit growth cone repulsive signalling pathway. In the nerve cord of elav mutant embryos, comm mRNA expression is strongly reduced in neurons, but not in midline glial cells. Furthermore, specific expression of an elav transgene in posterior neurons of each segment of an elav mutant nerve cord restores comm mRNA expression in these cells, as well as the formation of posterior commissures. Finally, forced expression of comm in specific commissural neuron subsets rescues the midline crossing defects of these neurons in elav mutant embryos, further indicating that elav acts cell autonomously on comm expression. |
| Databáze: | OpenAIRE |
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