Identification of novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in camel milk protein hydrolysates
| Autor: | Richard J. FitzGerald, Priti Mudgil, Sajid Maqsood, Alice B. Nongonierma, Sara Paolella |
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| Přispěvatelé: | University Program for Advanced Research (UPAR), EI, SFI |
| Rok vydání: | 2018 |
| Předmět: |
endocrine system
Camelus Protein Hydrolysates Dipeptidyl Peptidase 4 Drug Evaluation Preclinical Biology Dipeptidyl peptidase dipeptidyl peptidase IV inhibition Analytical Chemistry 0404 agricultural biotechnology Liquid chromatography–mass spectrometry Camel milk medicine Animals Peptide bond Computer Simulation Trypsin Amino Acid Sequence IC50 chemistry.chemical_classification Dipeptidyl-Peptidase IV Inhibitors Chromatography 04 agricultural and veterinary sciences General Medicine Milk Proteins 040401 food science Amino acid trypsin Milk Enzyme chemistry Biochemistry Cattle Peptides bioactive peptides camel milk proteins Chromatography Liquid Food Science medicine.drug |
| Zdroj: | Food Chemistry. 244:340-348 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2017.10.033 |
| Popis: | peer-reviewed Nine novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides (FLQY, FQLGASPY, ILDKEGIDY, ILELA, LLQLEAIR, LPVP, LQALHQGQIV, MPVQA and SPVVPF) were identified in camel milk proteins hydrolysed with trypsin. This was achieved using a sequential approach combining liquid chromatography tandem mass spectrometry (LC-MS/MS), qualitative/quantitative structure activity relationship (QSAR) and confirmatory studies with synthetic peptides. The most potent camel milk protein-derived DPP-IV inhibitory peptides, LPVP and MPVQA, had DPP-IV half maximal inhibitory concentrations (IC50) of 87.0 ± 3.2 and 93.3 ± 8.0 µM, respectively. DPP-IV inhibitory peptide sequences identified within camel and bovine milk protein hydrolysates generated under the same hydrolysis conditions differ. This was linked to differences in enzyme selectivity for peptide bond cleavage of camel and bovine milk proteins as well as dissimilarities in their amino acid sequences. Camel milk proteins contain novel DPP-IV inhibitory peptides which may play a role in the regulation of glycaemia in humans. ACCEPTED peer-reviewed |
| Databáze: | OpenAIRE |
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