Author Correction: M-protein based vaccine induces immunogenicity and protection from Streptococcus pyogenes when delivered on a high-density microarray patch (HD-MAP)
| Autor: | Paul V. Fahey, Jessica Dooley, Manisha Pandey, Alexandra C. I. Depelsenaire, Angus Forster, Simone Reynolds, Ainslie Calcutt, S. Ben Baker, Michael F. Good, Christine Wun, Cesar M. Jayashi Flores, Jamie-Lee Mills, Senthil Murugappan |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
2019-20 coronavirus outbreak Microarray Coronavirus disease 2019 (COVID-19) Myeloma protein Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Immunology Adaptive immunity Biology medicine.disease_cause lcsh:RC254-282 Microbiology Article Medical research medicine Pharmacology (medical) Author Correction Pharmacology Immunogenicity lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Virology Infectious Diseases Streptococcus pyogenes lcsh:RC581-607 Infection Biotechnology |
| Zdroj: | NPJ Vaccines npj Vaccines, Vol 5, Iss 1, Pp 1-1 (2020) |
| ISSN: | 2059-0105 |
| Popis: | We evaluated vaccination against Streptococcus pyogenes with the candidate vaccine, J8-DT, delivered by a high-density microarray patch (HD-MAP). We showed that vaccination with J8-DT eluted from a coated HD-MAP (J8-DT/HD-MAP), induced similar total IgG responses to that generated by vaccination with J8-DT adjuvanted with Alum (J8-DT/Alum). We evaluated the effect of dose reduction and the number of vaccinations on the antibody response profile of vaccinated mice. A reduction in the number of vaccinations (from three to two) with J8-DT/HD-MAP induced comparable antibody responses to three vaccinations with intramuscular J8-DT/Alum. Vaccine-induced protection against an S. pyogenes skin challenge was assessed. J8-DT/HD-MAP vaccination led to a significant reduction in the number of S. pyogenes colony forming units in skin (92.9%) and blood (100%) compared to intramuscular vaccination with unadjuvanted J8-DT. The protection profile was comparable to that of intramuscular J8-DT/Alum. J8-DT/HD-MAP induced a shift in the antibody isotype profile, with a bias towards Th1-related isotypes, compared to J8-DT/Alum (Th2 bias). Based on the results of this study, the use of J8-DT/HD-MAP should be considered in future clinical development and control programs against S. pyogenes. Furthermore, the innate characteristics of the technology, such as vaccine stability and increased coverage, ease of use, reduction of sharp waste and the potential reduction of dose may be advantageous compared to current vaccination methods. |
| Databáze: | OpenAIRE |
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