Vero-cell rabies vaccine produced using serum-free medium
| Autor: | Hisako Gondo Higashi, Regina Maria Mourão-Fuches, Rosana L. Paoli, Isaias Raw, N. M. Frazatti-Gallina, Elizabeth Juliana Ghiuro Valentini, Cosue Miyaki, Maria L.N. Silva |
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| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Virus Cultivation Biology medicine.disease_cause Antibodies Viral Culture Media Serum-Free Microbiology Mice Rabies vaccine Bioreactors Drug Stability Neutralization Tests Chlorocebus aethiops medicine Animals Mononegavirales Lyssavirus Vero Cells General Veterinary General Immunology and Microbiology Immunogenicity Rabies virus Public Health Environmental and Occupational Health Temperature Rhabdoviridae medicine.disease biology.organism_classification Virology Microspheres Infectious Diseases Rabies Vaccines Vero cell Molecular Medicine Virus Inactivation Rabies medicine.drug |
| Zdroj: | Vaccine. 23(4) |
| ISSN: | 0264-410X |
| Popis: | A new rabies vaccine was developed from Vero cells adhered to microcarriers, cultivated in a bioreactor in serum-free medium and infected with the PV/VERO-Paris rabies virus strain. The viral suspensions were concentrated by tangential filtration, purified by chromatography and inactivated with beta-propiolactone. In immunogenicity studies performed in mice immunized with three doses of the new vaccine (seven batches) and the commercial Verorab and HDCV, mean titers of neutralizing antibodies of 10.3-34.6, 6.54 and 9.36 IU/ml were found, respectively. The vaccine presented stability during 14 months at 2-8 degrees C, 30 days at 37 degrees C and 8 h at 45 degrees C. The use of serum-free medium facilitated the downstream process leading to residual cellular DNA values22.8 pg per dose of vaccine in all produced batches. The effective immunogenicity induced in mice by this vaccine, the degree of purity of the product, the high antigen yield and the reduction of the cost of the product due to the virus production and purification processes, makes this technology very important for countries where rabies presents a great public health problem. |
| Databáze: | OpenAIRE |
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