Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase
| Autor: | Maria Jose York-Duran, Barbara Rodriguez-Colinas, Francisco J. Plou, Empar Chenoll, Francisco M. Codoñer, María Jesús Martínez, Ester Pardo, Laura I. de Eugenio, Manuel Nieto-Domínguez |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Comunidad de Madrid |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine SCFAs Breast-fed Staphylococcus hominis Microorganism medicine.medical_treatment Oligosaccharides Xylose Biology Xylosidase 01 natural sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Xylobiose medicine Glycoside hydrolase Endo-1 4-beta Xylanases Prebiotic Hydrolysis General Medicine Xylan Lactic acid 030104 developmental biology Prebiotics chemistry Biochemistry Talaromyces XOS Xylanase Xylans Microbiome Bifidobacterium Food Science |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1873-7072 |
| Popis: | 34 p.-4 fig.-1 tab. A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae. The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan. A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components. The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms’ composition and organic acids profile in breast-fed child faeces fermentations. The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides. This work was carried out with funding from projects BIO2013-48779-C4-1-R, BIO2016-76601-C3-1-R and RTC-2014-1777-3 from MINECO, BIO2015-68387-R from MINECO/FEDER and S2013/MAE-2972 from Comunidad de Madrid. |
| Databáze: | OpenAIRE |
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