L-galactonate dehydratase is part of the fungal path for D-galacturonic acid catabolism
| Autor: | Merja Penttilä, Paula Jouhten, Peter Richard, Satu Kuorelahti, Hannu Maaheimo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Glycerol
Galactonate dehydratase Magnetic Resonance Spectroscopy Hypocrea Trichoderma reesei Biology Reductase Glyceraldehyde Microbiology Fungal Proteins chemistry.chemical_compound dehydratase Pyruvic Acid Cloning Molecular l-galactonate Molecular Biology Hydro-Lyases chemistry.chemical_classification Catabolism Hexuronic Acids catabolism biology.organism_classification Blotting Northern metabolomics fungal path Enzyme Biochemistry chemistry Dehydratase fungal gene expression D-Galacturonic acid Gene Deletion |
| Zdroj: | Kuorelahti, S, Jouhten, P, Maaheimo, H, Penttilä, M & Richard, P 2006, ' L-galactonate dehydratase is part of the fungal path for D-galacturonic acid catabolism ', Molecular Microbiology, vol. 61, no. 4, pp. 1060-1068 . https://doi.org/10.1111/j.1365-2958.2006.05294.x |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.2006.05294.x |
| Popis: | An L-galactonate dehydratase and the corresponding gene were identified from the mould Hypocrea jecorina (Trichoderma reesei). This novel enzyme converts L-galactonate to L-threo-3-deoxy-hexulosonate (2-keto-3-deoxy-L- galactonate). The enzyme is part of the fungal pathway for D-galacturonic acid catabolism, a pathway which is only partly known. It is the second enzyme of this pathway after the D-galacturonic acid reductase. L-galactonate dehydratase activity is present in H. jecorina cells grown on D-galacturonic acid but absent when other carbon sources are used for growth. A deletion of the L-galactonate dehydratase gene in H. jecorina results in a strain with no growth on D-galacturonic acid. The active enzyme was produced in the heterologous host Saccharomyces cerevisiae and characterized. It exhibited activity with L-galactonate and D-arabonate where the hydroxyl group of the C2 is in L- and the hydroxyl group of the C3 is in D-configuration in the Fischer projection. However, it did not exhibit activity with D-galactonate, D-gluconate, L-gulonate or D-xylonate where the hydroxyl groups of the C2 and C3 are in different configuration. |
| Databáze: | OpenAIRE |
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