Biocompatible scaffolds based on natural polymers for regenerative medicine
| Autor: | Salimgerey Adilov, Dana Akilbekova, Dmitriy Berillo, Madina Shaimerdenova |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
food.ingredient
02 engineering and technology 010402 general chemistry Borohydride Regenerative Medicine 01 natural sciences Biochemistry Gelatin Regenerative medicine Chitosan chemistry.chemical_compound Mice food Tissue engineering Structural Biology Materials Testing Zeta potential Animals Molecular Biology Tissue Scaffolds General Medicine Fibroblasts 021001 nanoscience & nanotechnology 3. Good health 0104 chemical sciences Dextran chemistry Chemical engineering NIH 3T3 Cells Glutaraldehyde 0210 nano-technology Cryogels |
| Zdroj: | International Journal of Biological Macromolecules |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.03.116 |
| Popis: | The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,3,3-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree ~2 times higher compared to the cryogels prepared with a two-step method i.e. reduced by borohydride. Scaffolds cross-linked by glutaraldehyde had about 40% viability, whereas nine various compositions of cryogels showed significantly higher viability (~80%) of fibroblast cells in vitro. The cryogels were obtained without using the harmful compounds and therefore can be used straightforward as biocompatible and biodegradable scaffolds for the cell culturing purposes and other biomedical applications. |
| Databáze: | OpenAIRE |
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