Influence of CNTRENE® C100LM carbon nanotube material on the growth and regulation of Escherichia coli
| Autor: | Adam K. Wanekaya, Geoffrey Manani, Paul Schweiger, Brittany L. Twibell, Molly Duszynski, Kalie M. Somerville |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Microorganism
Cytotoxicity Nanoparticle lcsh:Medicine 02 engineering and technology Carbon nanotube 010402 general chemistry Cell morphology medicine.disease_cause 01 natural sciences General Biochemistry Genetics and Molecular Biology law.invention Microbiology Life cycle assessment law medicine Escherichia coli Minimum inhibitory concentration Chemistry General Neuroscience lcsh:R General Medicine 021001 nanoscience & nanotechnology Decomposition 0104 chemical sciences Biophysics Nanoparticles Gene expression Electron microscope 0210 nano-technology General Agricultural and Biological Sciences |
| Zdroj: | PeerJ, Vol 5, p e3721 (2017) |
| ISSN: | 2167-8359 |
| Popis: | The growing use of carbon nanotubes (CNTs) in industrial and consumer products raises important questions about their environmental fate and impact on prokaryotes. In the environment, CNTs are exposed to a variety of conditions (e.g., UV light) that could lead to decomposition and changes in their chemical properties. Therefore, the potential cytotoxic effect of both pristine and artificially aged carboxyl functionalized CNTRENE®C100LM CNTmaterial at neutral and acidic conditions onEscherichia coliK12 was analyzed using a minimal inhibitory concentration (MIC) assay, which also allowed monitoring of non-lethal growth effects. However, there were no observable MIC or significant changes in growth behavior inE. coliK12 when exposed to pristine or aged CNTs. Exposure to pristine CNTRENE®C100LM CNT material did not appear to influence cell morphology or damage the cells when examined by electron microscopy. In addition, RNA sequencing revealed no observable regulatory changes in typical stress response pathways. This is surprising considering that previous studies have claimed high cytotoxicity of CNTs, including carboxyl functionalized single-walled CNTs, and suggest that other factors such as trace heavy metals or other impurities are likely responsible for many of the previously reported cytotoxicity inE. coliand possibly other microorganisms. |
| Databáze: | OpenAIRE |
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