Epigallocatechin gallate increase the prostacyclin production of bovine aortic endothelial cells
| Autor: | Takanori Hishinuma, Michinao Mizugaki, Tohru Yamazaki, Fumiaki Ishizawa |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Physiology
Prostacyclin Epigallocatechin gallate Pharmacology complex mixtures Biochemistry Catechin Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Fibrinolytic Agents Gallic Acid medicine Animals heterocyclic compounds Gallic acid Cells Cultured Arachidonic Acid Dose-Response Relationship Drug Molecular Structure food and beverages Thromboxanes Cell Biology Eicosapentaenoic acid Epoprostenol Epicatechin gallate Pyrogallol chemistry Eicosapentaenoic Acid cardiovascular system lipids (amino acids peptides and proteins) Arachidonic acid Cattle Endothelium Vascular Platelet Aggregation Inhibitors medicine.drug |
| Zdroj: | Prostaglandinsother lipid mediators. 62(2) |
| ISSN: | 1098-8823 |
| Popis: | We describe the effect of (-) epigallocatechin gallate (EGCg), one of catechins known in tea, on the prostacyclin (PGI) production by bovine aortic endothelial cells. The amounts of 6-keto-PGF(1alpha) and Delta(17)-6-keto-PGF(1alpha), stable metabolites of PGI(2) and PGI(3), released in culture medium were measured using gas chromatography/selected ion monitoring (GC/SIM). The prostacyclin production of endothelial cells was increased by EGCg in a dose- and time-dependent manner. The effect by EGCg was stronger than any other catechins (catechin, epicatechin, epigallocatechin, and epicatechin gallate). When endothelial cells incubated with EGCg and arachidonic acid (AA) or eicosapentaenoic acid (EPA), PGI(2), and PGI(3) production were increased greater than those incubated with AA or EPA alone. Furthermore, gallic acid, that also has a pyrogallol structure, increased PGI(2) production. These observations indicate that catechins increase the prostacyclin production and that the pyrogallol structure is significant to this function. |
| Databáze: | OpenAIRE |
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