Colocalization of centromeric and replicative functions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica
| Autor: | D M Ogrydziak, Philippe Fournier, C Feynerol, A M Ribet, D Yaver, B Kudla, M. Chasles, A Peito, A Abbas, Jian-Wu Xuan |
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| Rok vydání: | 1993 |
| Předmět: |
DNA Replication
Autonomously replicating sequence Centromere Genes Fungal Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae Transformation Genetic Plasmid Sequence Homology Nucleic Acid Consensus sequence Sister chromatids Cloning Molecular DNA Fungal Genetics Multidisciplinary Base Sequence biology Yarrowia biology.organism_classification Saccharomycetales Chromosomes Fungal Chromosome breakage Gene Deletion Plasmids Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 90:4912-4916 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.90.11.4912 |
| Popis: | Two sequences (ARS18 and ARS68) displaying autonomous replication activity were previously cloned in the yeast Yarrowia lipolytica. The smallest fragment (1-1.3 kb) required for extrachromosomal replication of a plasmid is significantly larger in Y. lipolytica than in Saccharomyces cerevisiae. Neither autonomously replicating sequence (ARS) is homologous with known ARS or centromere (CEN) consensus sequences. They share short regions of sequence similarity with each other. These ARS fragments also contain Y. lipolytica centromeres: (i) integration of marker genes at the ARS loci results in a CEN-linked segregation of the markers, (ii) an ARS on a plasmid largely maintains sister chromatid attachment in meiosis I, and (iii) integration of these sequences at the LEU2 locus leads to chromosome breakage. Deletions performed on ARS18 show that CEN and ARS functions can be physically separated, but both are needed to establish a replicating plasmid. |
| Databáze: | OpenAIRE |
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