Pheromone-encoding mRNA is transported to the yeast mating projection by specific RNP granules
| Autor: | Mordechai Choder, Saray Dover-Biterman, Michael Shmoish, Stella Aronov, Lea Duek, Edith Suss-Toby |
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| Rok vydání: | 2015 |
| Předmět: |
Mating type
Saccharomyces cerevisiae Proteins Lipoproteins Saccharomyces cerevisiae Cycloheximide Biology Cytoplasmic Granules Cell Membrane Structures Article Pheromones chemistry.chemical_compound MRNA transport RNA Messenger reproductive and urinary physiology Research Articles Ribonucleoprotein Genetics Messenger RNA Biological Transport Translation (biology) Cell Biology biology.organism_classification Cell biology Ribonucleoproteins chemistry Mating of yeast behavior and behavior mechanisms Cell Surface Extensions |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201408045 |
| Popis: | In response to mating pheromone, the yeast MFA2 mRNA is transported to the tip of the mating projection as an RNP granule and translated; integrity of the granules is required for normal mRNA transport and for the mating process. Association of messenger RNAs with large complexes such as processing bodies (PBs) plays a pivotal role in regulating their translation and decay. Little is known about other possible functions of these assemblies. Exposure of haploid yeast cells, carrying mating type “a,” to “α pheromone” stimulates polarized growth resulting in a “shmoo” projection; it also induces synthesis of “a pheromone,” encoded by MFA2. In this paper, we show that, in response to α pheromone, MFA2 mRNA is assembled with two types of granules; both contain some canonical PB proteins, yet they differ in size, localization, motility, and sensitivity to cycloheximide. Remarkably, one type is involved in mRNA transport to the tip of the shmoo, whereas the other—in local translation in the shmoo. Normal assembly of these granules is critical for their movement, localization, and for mating. Thus, MFA2 mRNAs are transported to the shmoo tip, in complex with PB-like particles, where they are locally translated. |
| Databáze: | OpenAIRE |
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