UV-A-induced expression of GroEL in the UV-A-resistant marine cyanobacterium Oscillatoria sp. NKBG 091600
| Autor: | Akira Yamazawa, Daisuke Takeda, Haruko Takeyama, Tadashi Matsunaga |
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| Rok vydání: | 1999 |
| Předmět: |
Transcription
Genetic Ultraviolet Rays Sequence analysis Operon Molecular Sequence Data Cyanobacteria Radiation Tolerance Microbiology Gene expression Consensus sequence SOS response Oscillatoria Base Sequence biology Nucleic acid sequence Chaperonin 60 Gene Expression Regulation Bacterial Sequence Analysis DNA Blotting Northern biology.organism_classification GroEL enzymes and coenzymes (carbohydrates) Biochemistry bacteria Electrophoresis Polyacrylamide Gel |
| Zdroj: | Microbiology. 145:949-954 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/13500872-145-4-949 |
| Popis: | The authors have examined the response to UV-A irradiation of the UV-A-resistant marine cyanobacterium Oscillatoria sp. NKBG 091600, which produces the UV-A-absorbing compound biopterin glucoside. The expression of a 60 kDa protein was markedly induced at 500 min after UV-A irradiation. This protein was identified by N-terminal amino acid sequence analysis as GroEL. Northern blot analysis demonstrated that GroEL synthesis was controlled by UV-A at the transcriptional level. A CIRCE element and a putative SOS consensus sequence were found upstream of the groESL operon, overlapping two putative promoter sequences. Primer extension analysis revealed that groESL transcription in UV-A-induced cells starts from the proximal promoter overlapped by the SOS consensus sequence. This indicates that an SOS response regulation is instrumental in UV-A-induced GroEL expression of Oscillatoria sp. NKBG 091600. Furthermore, this UV-A-inducible GroEL may function to upregulate biopterin glucoside biosynthesis, thereby allowing growth under UV-A irradiation. |
| Databáze: | OpenAIRE |
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