Optimized Gas Chromatography-Tandem Mass Spectrometry for 1,1'-sulfonylbis[2-(methylthio) ethane] Quantification in Human Urine

Autor:	Mesut Ortatatli, Sermet Sezigen, Rusen Koray Eyison, Levent Kenar
Rok vydání:	2019
Předmět:	Analyte Lyases Urine Mass spectrometry 01 natural sciences Sensitivity and Specificity Gas Chromatography-Mass Spectrometry Analytical Chemistry Cartridge chemistry.chemical_compound Tandem Mass Spectrometry Mustard Gas Humans Chemical Warfare Agents Retrospective Studies Ethane Chromatography Gas Chromatography/Tandem Mass Spectrometry 010405 organic chemistry Chemistry 010401 analytical chemistry Extraction (chemistry) Sulfur mustard General Medicine Environmental Exposure 0104 chemical sciences Biomarker (petroleum) Biomarkers
Zdroj:	Journal of chromatographic science. 57(5)
ISSN:	1945-239X
Popis:	Sulfur mustard (SM) which is a bifunctional alkylating vesicant is one of the mostly used chemical warfare agent in First World War and the Iran–Iraq War. β-Lyase metabolites of SM especially 1,1′-sulfonylbis[2-(methylthio)ethane] (SBMTE) is an unequivocal biomarker of the exposure. An optimized gas chromatography-tandem mass spectrometry method was developed and validated for the retrospective detection of SBMTE in human urine. Urine samples were treated with acidic titanium trichloride to reduce β-lyase metabolites to the single analyte SBMTE. After neutralization and precipitation, SBMTE was extracted from urine by C8 solid-phase extraction cartridge and analyzed in the multiple-reaction monitoring mode. The lower limit of quantification was 1 ng/mL with relative standard deviation of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e21562998272523c0dfec33dae815eb https://pubmed.ncbi.nlm.nih.gov/30882871 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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