Inoculation of Balb/c mice with live attenuated tachyzoites protects against a lethal challenge ofNeospora caninum
| Autor: | D. Buxton, Francesca Chianini, Stephen E. Wright, Paul M. Bartley, E.A. Innes |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Protozoan Vaccines
Time Factors Necrosis Ratón Antibodies Protozoan Virulence Antigens Protozoan Vaccines Attenuated BALB/c Mice Neospora Immunity DNA Ribosomal Spacer medicine Animals Brain Diseases Mice Inbred BALB C biology Coccidiosis Body Weight Brain DNA Protozoan biology.organism_classification Virology Neospora caninum Disease Models Animal Infectious Diseases biology.protein Female Immunization Animal Science and Zoology Parasitology Antibody medicine.symptom |
| Zdroj: | Parasitology. 135:13-21 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182007003526 |
| Popis: | SUMMARYNeospora caninumtachyzoites attenuated through passage in tissue culture were tested for their ability to induce protective immunity against a lethal challenge dose of parasites. Balb/c mice were each inoculated with either 1×106live virulent tachyzoites (Group 1) or 1×106live attenuated tachyzoites (Group 2), while (Group 3) received a control inoculum. All mice were each challenged 28 days later with 5×106virulent parasites. Histopathological lesions in the brains including necrosis and microgliosis were observed following post-mortem on day 28 post-challenge (p.c.) in 71% of Group 1 and 56% of Group 2. Immunohistochemistry (IHC) of these lesions showed tachyzoites andNeosporaantigens to be associated with moderate brain lesions in 17% of Group 1, while in 11% of Group 2N. caninumtissue cysts were detected, but these were not associated with lesions, Parasite DNA was detected by PCR in the brains of 86% of mice in Group 1 and 56% of mice in Group 2. Following challenge the mice in Group 3 showed high morbidity and 100% mortality within 17 days p.c. Positive IHC forN. caninumwas seen in 88% of the Group 3 mice and parasite DNA was detected in all brain samples. This study shows that it is possible to protect against a lethal challenge ofN. caninumthrough inoculation with attenuated or virulent tachyzoites. However, more severe pathology developed in mice initially inoculated with virulent parasites following a secondary challenge, compared to mice initially inoculated with attenuated parasites. |
| Databáze: | OpenAIRE |
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