Is cytokeratin immunoreactivity useful in the diagnosis of short-segment Barrett??s oesophagus in Korea?
| Autor: | Jin Yong Kim, Sang Woo Lee, Hyung Joon Yim, Jin Hai Hyun, Youngsun Kim, Rok Son Choung, Hong Sik Lee, Chul-Hwan Kim, Ho Sang Ryu, Jai Hyun Choi, Young Tae Bak, Chi Wook Song, Dae Soo Kim |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Biopsy Keratin-20 Sensitivity and Specificity digestive system Gastroenterology Barrett Esophagus Cytokeratin Intermediate Filament Proteins Internal medicine medicine Humans Aged Metaplasia Hepatology medicine.diagnostic_test Esophageal disease business.industry Stomach Keratin-7 Squamocolumnar Junction Intestinal metaplasia Cardia Middle Aged medicine.disease digestive system diseases Staining surgical procedures operative medicine.anatomical_structure Keratins Immunohistochemistry Female Esophagoscopy business Biomarkers |
| Zdroj: | European Journal of Gastroenterology & Hepatology. 17:611-616 |
| ISSN: | 0954-691X |
| DOI: | 10.1097/00042737-200506000-00004 |
| Popis: | Background Cytokeratin 7/20 staining has been reported to be helpful in diagnosing Barrett's oesophagus and gastric intestinal metaplasia. However, this is still a matter of some controversy. Objective To determine the diagnostic usefulness of cytokeratin 7/20 immunostaining for short-segment Barrett's oesophagus in Korea. Methods In patients with Barrett's oesophagus, diagnosed endoscopically, at least two biopsy specimens were taken from just below the squamocolumnar junction. If goblet cells were found histologically with alcian blue staining, cytokeratin 7/20 immunohistochemical stains were performed. Intestinal metaplasia at the cardia was diagnosed whenever biopsy specimens taken from within 2 cm below the oesophagogastric junction revealed intestinal metaplasia. Barrett's cytokeratin 7/20 pattern was defined as cytokeratin 20 positivity in only the superficial gland, combined with cytokeratin 7 positivity in both the superficial and deep glands. Results Barrett's cytokeratin 7/20 pattern was observed in 28 out of 36 cases (77.8%) with short-segment Barrett's oesophagus, 11 out of 28 cases (39.3%) with intestinal metaplasia at the cardia, and nine out of 61 cases (14.8%) with gastric intestinal metaplasia. The sensitivity and specificity of Barrett's cytokeratin 7/20 pattern were 77.8 and 77.5%, respectively. Conclusion Barrett's cytokeratin 7/20 pattern can be a useful marker for the diagnosis of short-segment Barrett's oesophagus, although the false positive or false negative rate is approximately 25%. |
| Databáze: | OpenAIRE |
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