Miniaturized direct on air sampling filter quantification of pollen allergens
| Autor: | Leif Holmquist, Olof Vesterberg |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Air sampling
Biophysics Poaceae medicine.disease_cause Biochemistry Trees chemistry.chemical_compound Pollen Grass pollen otorhinolaryngologic diseases medicine Polytetrafluoroethylene Immunoassay Detection limit Miniaturization Chromatography medicine.diagnostic_test Air food and beverages Substrate (chemistry) Membranes Artificial Allergens Dioxetane Filter (aquarium) chemistry Luminescent Measurements Regression Analysis Filtration |
| Zdroj: | Journal of Biochemical and Biophysical Methods. 42:111-114 |
| ISSN: | 0165-022X |
| DOI: | 10.1016/s0165-022x(99)00050-0 |
| Popis: | A recently in this journal reported luminescence immunoassay for the direct quantification of birch and grass pollen allergens on air sampling filters, DOSIS, has been miniaturized. By means of a commercially avaliable chlorinated analogue of the previously used 1,2 dioxetane phosphate derivative as enzyme substrate, the air sampling filter diameter could be reduced from 25 mm to 13 mm. The procedure leads to a more than twenty times reduction of the previously reported limit of quantification for the grass pollen allergen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect